Detailed information of TA system TA049601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1203516..1204165
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1A383
• Locus tag: EC036_RS05660
• Protein ID: WP_013097692.1
• Coordinates: 1203803..1204165 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC036_RS05655
• Protein ID: WP_038418841.1
• Coordinates: 1203516..1203815 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS05645	1199928..1201901	+	1974	WP_038418838.1	PhoX family phosphatase	-
EC036_RS05650	1202004..1203392	+	1389	WP_038418840.1	phenylalanine transporter	-
EC036_RS05655	1203516..1203815	-	300	WP_038418841.1	XRE family transcriptional regulator	Antitoxin
EC036_RS05660	1203803..1204165	-	363	WP_013097692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EC036_RS05665	1204371..1205609	+	1239	WP_029882613.1	MFS transporter	-
EC036_RS05670	1205625..1206650	+	1026	WP_038418842.1	sugar phosphate isomerase/epimerase	-
EC036_RS05675	1206643..1207785	+	1143	WP_038418844.1	Gfo/Idh/MocA family oxidoreductase	-
EC036_RS05680	1207861..1208832	+	972	WP_038418845.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13994.00 Da
• Isoelectric Point: 7.3235

>T49601 WP_013097692.1 NZ_CP009756:c1204165-1203803 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Nucleotide

• Length: 363 bp

>T49601 NZ_CP009756:c1204165-1203803 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10861.59 Da
• Isoelectric Point: 8.6152

>AT49601 WP_038418841.1 NZ_CP009756:c1203815-1203516 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLRDELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Nucleotide

• Length: 300 bp

>AT49601 NZ_CP009756:c1203815-1203516 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTAATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGATGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACAGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTAACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA

Structures

Toxin

• AlphaFold DB: A0A5E1A383