Detailed information of TA system TA049450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3448956..3449497
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: EO53_RS17190
• Protein ID: WP_000615983.1
• Coordinates: 3449219..3449497 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: EO53_RS17185
• Protein ID: WP_000729703.1
• Coordinates: 3448956..3449216 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO53_RS17165	3444631..3445416	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
EO53_RS17170	3445388..3447100	+	1713	Protein_3273	flagellar biosynthesis protein FlhA	-
EO53_RS17175	3447324..3447821	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
EO53_RS17180	3447997..3448746	-	750	WP_000056849.1	C40 family peptidase	-
EO53_RS17185	3448956..3449216	+	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
EO53_RS17190	3449219..3449497	+	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
EO53_RS17195	3449653..3450393	+	741	WP_001225679.1	murein L,D-transpeptidase	-
EO53_RS17200	3450364..3451131	-	768	WP_000333380.1	class II glutamine amidotransferase	-
EO53_RS17205	3451337..3451915	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3398438..3473844	75406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T49450 WP_000615983.1 NZ_CP009685:3449219-3449497 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T49450 NZ_CP009685:3449219-3449497 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT49450 WP_000729703.1 NZ_CP009685:3448956-3449216 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT49450 NZ_CP009685:3448956-3449216 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716