Detailed information of TA system TA049449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3442759..3443453
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: EO53_RS17150
• Protein ID: WP_001263489.1
• Coordinates: 3442759..3443157 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EO53_RS17155
• Protein ID: WP_000554758.1
• Coordinates: 3443160..3443453 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3438348..3438428	-	81	NuclAT_12	-	-
-	3438348..3438428	-	81	NuclAT_12	-	-
-	3438348..3438428	-	81	NuclAT_12	-	-
-	3438348..3438428	-	81	NuclAT_12	-	-
EO53_RS17125	3439024..3439482	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
EO53_RS17130	3439743..3441199	+	1457	Protein_3265	cytosol nonspecific dipeptidase	-
EO53_RS17135	3441256..3441777	-	522	Protein_3266	peptide chain release factor H	-
EO53_RS17140	3441773..3441979	-	207	Protein_3267	RtcB family protein	-
EO53_RS17145	3442297..3442749	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
EO53_RS17150	3442759..3443157	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EO53_RS17155	3443160..3443453	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EO53_RS17160	3443505..3444560	-	1056	WP_001226164.1	DNA polymerase IV	-
EO53_RS17165	3444631..3445416	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
EO53_RS17170	3445388..3447100	+	1713	Protein_3273	flagellar biosynthesis protein FlhA	-
EO53_RS17175	3447324..3447821	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3398438..3473844	75406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T49449 WP_001263489.1 NZ_CP009685:c3443157-3442759 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T49449 NZ_CP009685:c3443157-3442759 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT49449 WP_000554758.1 NZ_CP009685:c3443453-3443160 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT49449 NZ_CP009685:c3443453-3443160 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3