49434

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1610438..1610940 Replicon chromosome
Accession NZ_CP009685
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EO53_RS07820 Protein ID WP_000767829.1
Coordinates 1610686..1610940 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EO53_RS07815 Protein ID WP_001259255.1
Coordinates 1610438..1610689 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO53_RS07795 1605616..1606683 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EO53_RS07800 1606683..1607753 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
EO53_RS07805 1607750..1609054 - 1305 WP_000009594.1 histidinol dehydrogenase -
EO53_RS07810 1609060..1609959 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EO53_RS23690 1610105..1610155 - 51 WP_001364200.1 his operon leader peptide -
EO53_RS07815 1610438..1610689 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EO53_RS07820 1610686..1610940 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EO53_RS07825 1611023..1611847 + 825 WP_000754737.1 SDR family oxidoreductase -
EO53_RS07830 1611893..1612822 + 930 WP_000803351.1 LysR family transcriptional regulator -
EO53_RS25635 1613037..1613099 + 63 WP_010723108.1 membrane protein YoeI -
EO53_RS07835 1613089..1614447 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EO53_RS07840 1614626..1615684 + 1059 WP_000492339.1 transport protein YeeE -
EO53_RS07845 1615698..1615925 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T49434 WP_000767829.1 NZ_CP009685:1610686-1610940 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T49434 NZ_CP009685:1610686-1610940 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT49434 WP_001259255.1 NZ_CP009685:1610438-1610689 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT49434 NZ_CP009685:1610438-1610689 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References