Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 531613..532306 Replicon chromosome
Accession NZ_CP009685
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag EO53_RS02675 Protein ID WP_000415584.1
Coordinates 531613..531909 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag EO53_RS02680 Protein ID WP_000650107.1
Coordinates 531911..532306 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO53_RS02640 526701..527015 - 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -
EO53_RS02645 527046..527627 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
EO53_RS02650 527946..528278 + 333 WP_000917684.1 DUF2645 family protein -
EO53_RS02655 528324..529673 - 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
EO53_RS02660 529670..530329 - 660 WP_001221495.1 two-component system response regulator QseB -
EO53_RS02665 530481..530873 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
EO53_RS02670 530926..531408 + 483 WP_000183505.1 transcriptional regulator -
EO53_RS02675 531613..531909 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
EO53_RS02680 531911..532306 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
EO53_RS02685 532439..534046 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
EO53_RS02690 534184..536442 + 2259 WP_001281881.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T49429 WP_000415584.1 NZ_CP009685:531613-531909 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T49429 NZ_CP009685:531613-531909 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT49429 WP_000650107.1 NZ_CP009685:531911-532306 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT49429 NZ_CP009685:531911-532306 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References