Detailed information of TA system TA049429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 531613..532306
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: EO53_RS02675
• Protein ID: WP_000415584.1
• Coordinates: 531613..531909 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: EO53_RS02680
• Protein ID: WP_000650107.1
• Coordinates: 531911..532306 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO53_RS02640	526701..527015	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
EO53_RS02645	527046..527627	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
EO53_RS02650	527946..528278	+	333	WP_000917684.1	DUF2645 family protein	-
EO53_RS02655	528324..529673	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
EO53_RS02660	529670..530329	-	660	WP_001221495.1	two-component system response regulator QseB	-
EO53_RS02665	530481..530873	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
EO53_RS02670	530926..531408	+	483	WP_000183505.1	transcriptional regulator	-
EO53_RS02675	531613..531909	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
EO53_RS02680	531911..532306	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
EO53_RS02685	532439..534046	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
EO53_RS02690	534184..536442	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T49429 WP_000415584.1 NZ_CP009685:531613-531909 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T49429 NZ_CP009685:531613-531909 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT49429 WP_000650107.1 NZ_CP009685:531911-532306 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT49429 NZ_CP009685:531911-532306 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65