Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2079356..2079858 Replicon chromosome
Accession NZ_CP009644
Organism Escherichia coli ER2796 strain K-12 ER2796

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ER2796_RS10495 Protein ID WP_000767829.1
Coordinates 2079356..2079610 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ER2796_RS10500 Protein ID WP_001259255.1
Coordinates 2079607..2079858 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER2796_RS10470 2074371..2074598 - 228 WP_000234896.1 sulfurtransferase TusA -
ER2796_RS10475 2074612..2075670 - 1059 WP_000492339.1 transport protein YeeE -
ER2796_RS10480 2075849..2077207 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ER2796_RS25360 2077197..2077259 - 63 WP_010723108.1 membrane protein YoeI -
ER2796_RS10485 2077474..2078403 - 930 WP_000803351.1 LysR family transcriptional regulator -
ER2796_RS10490 2078449..2079273 - 825 WP_000754737.1 SDR family oxidoreductase -
ER2796_RS10495 2079356..2079610 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ER2796_RS10500 2079607..2079858 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ER2796_RS23680 2080141..2080191 + 51 WP_001364200.1 his operon leader peptide -
ER2796_RS10505 2080337..2081200 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
ER2796_RS10510 2081206..2082510 + 1305 WP_000009594.1 histidinol dehydrogenase -
ER2796_RS10515 2082507..2083577 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ER2796_RS10520 2083577..2084644 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T49374 WP_000767829.1 NZ_CP009644:c2079610-2079356 [Escherichia coli ER2796]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T49374 NZ_CP009644:c2079610-2079356 [Escherichia coli ER2796]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT49374 WP_001259255.1 NZ_CP009644:c2079858-2079607 [Escherichia coli ER2796]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT49374 NZ_CP009644:c2079858-2079607 [Escherichia coli ER2796]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References