Detailed information of TA system TA049336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 6633..7156
• Replicon: plasmid unamed
• Accession: NZ_CP009624
• Organism: Staphylococcus agnetis strain 908

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EP23_RS24380
• Protein ID: WP_060552542.1
• Coordinates: 6890..7156 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: EP23_RS24375
• Protein ID: WP_060552541.1
• Coordinates: 6633..6890 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP23_RS24350	2344..2985	-	642	Protein_2	protein rep	-
EP23_RS24355	3093..3560	+	468	WP_156407422.1	helix-turn-helix domain-containing protein	-
EP23_RS24360	4331..4762	+	432	WP_031838157.1	hypothetical protein	-
EP23_RS24365	5133..5633	+	501	WP_060552539.1	hypothetical protein	-
EP23_RS24370	5783..6052	+	270	WP_060552540.1	hypothetical protein	-
EP23_RS24375	6633..6890	+	258	WP_060552541.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EP23_RS24380	6890..7156	+	267	WP_060552542.1	Txe/YoeB family addiction module toxin	Toxin
EP23_RS24385	7587..8141	+	555	WP_001062591.1	recombinase family protein	-
EP23_RS24390	8152..8400	+	249	WP_015379476.1	helix-turn-helix transcriptional regulator	-
EP23_RS24395	9236..9919	-	684	WP_000277644.1	CPBP family intramembrane metalloprotease	-
EP23_RS24400	10094..10432	-	339	WP_001022435.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EP23_RS24405	10429..10698	-	270	WP_001129937.1	hypothetical protein	-
EP23_RS24410	10999..11592	-	594	WP_060552544.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28987	28987
	-	flank	IS/Tn	-	-	10999..11592	593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10552.06 Da
• Isoelectric Point: 10.1699

>T49336 WP_060552542.1 NZ_CP009624:6890-7156 [Staphylococcus agnetis]
MNQYTVKIKNSAKSDLKKLKRSHLKEQFIEIVETLKSNPYEASQSFEKLEPKNLKRYSRRINHQHRVVYTVDDQNKEVLI
LAAWSQYE

Nucleotide

• Length: 267 bp

>T49336 NZ_CP009624:6890-7156 [Staphylococcus agnetis]
ATGAATCAATATACTGTAAAAATAAAGAATTCAGCAAAATCTGATTTAAAAAAATTAAAACGCTCTCACTTAAAAGAACA
ATTTATTGAAATTGTGGAAACATTAAAAAGTAATCCGTATGAAGCTTCACAATCATTTGAGAAGCTAGAGCCTAAAAATT
TAAAGAGATATTCAAGAAGAATTAATCATCAACATAGAGTTGTTTATACTGTAGATGATCAGAATAAAGAAGTTTTAATA
TTAGCTGCATGGTCACAATATGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9873.98 Da
• Isoelectric Point: 5.1007

>AT49336 WP_060552541.1 NZ_CP009624:6633-6890 [Staphylococcus agnetis]
MNITNPTDARKDFYQLLKNVNSNHEPIYISGKNTENNAVIIGLKDWKSIQETMYLESTGTMDKVRERERDTSGKTNIEDI
DWHSL

Nucleotide

• Length: 258 bp

>AT49336 NZ_CP009624:6633-6890 [Staphylococcus agnetis]
ATGAATATCACAAACCCAACAGATGCTAGAAAAGACTTTTACCAATTGTTAAAAAACGTAAACAGCAATCATGAGCCTAT
TTATATTAGTGGTAAAAATACTGAAAATAATGCTGTTATTATTGGCTTAAAAGATTGGAAGAGTATTCAAGAAACTATGT
ACTTAGAATCAACAGGAACAATGGATAAAGTGAGAGAACGAGAGCGAGATACTAGCGGAAAAACGAATATAGAAGACATT
GATTGGCATAGCCTTTAA