Detailed information of TA system TA049324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1257488..1258127
• Replicon: chromosome
• Accession: NZ_CP009610
• Organism: Haemophilus influenzae strain Hi375

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: NF38_RS06150
• Protein ID: WP_005650215.1
• Coordinates: 1257488..1257793 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NF38_RS06155
• Protein ID: WP_005650217.1
• Coordinates: 1257804..1258127 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NF38_RS06130	1252917..1254956	-	2040	WP_038441065.1	excinuclease ABC subunit B	-
NF38_RS06140	1255572..1256540	-	969	WP_038441067.1	zinc transporter permease subunit ZevB	-
NF38_RS06145	1256543..1257163	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
NF38_RS06150	1257488..1257793	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NF38_RS06155	1257804..1258127	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
NF38_RS06160	1258290..1259960	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
NF38_RS06165	1260022..1260363	+	342	WP_005652996.1	SirB2 family protein	-
NF38_RS06170	1260368..1262338	+	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
NF38_RS09415	1262335..1262508	+	174	WP_162627973.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T49324 WP_005650215.1 NZ_CP009610:1257488-1257793 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T49324 NZ_CP009610:1257488-1257793 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT49324 WP_005650217.1 NZ_CP009610:1257804-1258127 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT49324 NZ_CP009610:1257804-1258127 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70