Detailed information of TA system TA049312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 692703..693360
• Replicon: chromosome
• Accession: NZ_CP009601
• Organism: Burkholderia thailandensis 2002721643

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: BG87_RS03105
• Protein ID: WP_009910242.1
• Coordinates: 693028..693360 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BG87_RS29715
• Protein ID: WP_011402654.1
• Coordinates: 692703..693020 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG87_RS03085	689006..689797	+	792	WP_009910252.1	ethanolamine ammonia-lyase subunit EutC	-
BG87_RS29705	689730..690278	-	549	WP_080511571.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
BG87_RS03090	690244..690804	-	561	Protein_615	LysR family transcriptional regulator	-
BG87_RS03095	690884..691849	+	966	Protein_616	IS256 family transposase	-
BG87_RS29710	691963..692451	-	489	Protein_617	site-specific integrase	-
BG87_RS29715	692703..693020	-	318	WP_011402654.1	helix-turn-helix domain-containing protein	Antitoxin
BG87_RS03105	693028..693360	-	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG87_RS03110	693402..693944	-	543	WP_102860125.1	site-specific integrase	-
BG87_RS32945	693994..694751	+	758	WP_099005207.1	IS5 family transposase	-
BG87_RS03125	695152..695931	-	780	WP_011402651.1	hypothetical protein	-
BG87_RS03130	696390..696971	-	582	WP_043300356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T49312 WP_009910242.1 NZ_CP009601:c693360-693028 [Burkholderia thailandensis 2002721643]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T49312 NZ_CP009601:c693360-693028 [Burkholderia thailandensis 2002721643]
ATGAAATTTGATTGGACGGTCGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTCGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAATTCGGGGCGGATCTACGGATGCCGCATTCCAGAGCAATGGGTAACG
GGCTGTTTGAGTTGCGCCCCAAGGGTAGGGAAGGCATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11277.80 Da
• Isoelectric Point: 10.2764

>AT49312 WP_011402654.1 NZ_CP009601:c693020-692703 [Burkholderia thailandensis 2002721643]
VKRVRAEGFNPVPHTADDTARLLAGRNVKAAYDALEDEYTALRAILSARRDAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT49312 NZ_CP009601:c693020-692703 [Burkholderia thailandensis 2002721643]
GTGAAGCGTGTTCGCGCTGAGGGATTCAATCCGGTTCCGCATACGGCGGACGACACGGCGCGCCTGCTCGCCGGTCGCAA
TGTCAAGGCGGCGTATGACGCGTTGGAGGATGAGTACACCGCGTTGCGCGCTATCTTGTCTGCACGGCGCGATGCTGGTC
TGACACAGGCTCAGATTGCTGAGCGGATGGGTACGACCGCATCGGCGGTCTCGCGGCTTGAGGCTTCGTTGTCGAGTGAA
AAGCATTCACCATCATTTGCGACGCTGCGTAAATACGCGGCTGCATGTGGTAAGCGGCTTGTGATTTCGTTTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8