Detailed information of TA system TA049295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 459281..459938
• Replicon: chromosome
• Accession: NZ_CP009585
• Organism: Burkholderia pseudomallei strain PHLS 112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DP45_RS02115
• Protein ID: WP_009972517.1
• Coordinates: 459606..459938 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DP45_RS31285
• Protein ID: WP_009972518.1
• Coordinates: 459281..459598 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP45_RS02095	455265..456134	-	870	WP_004189208.1	patatin-like phospholipase family protein	-
DP45_RS02100	456172..456576	-	405	WP_004200732.1	PaaI family thioesterase	-
DP45_RS38360	457191..457853	-	663	WP_133670743.1	hypothetical protein	-
DP45_RS31280	457874..459082	-	1209	WP_009972519.1	DUF262 domain-containing protein	-
DP45_RS31285	459281..459598	-	318	WP_009972518.1	helix-turn-helix domain-containing protein	Antitoxin
DP45_RS02115	459606..459938	-	333	WP_009972517.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DP45_RS02130	461511..461993	+	483	WP_004200731.1	DUF2778 domain-containing protein	-
DP45_RS02135	461990..462274	+	285	WP_004523107.1	hypothetical protein	-
DP45_RS02140	462348..463439	-	1092	WP_038724261.1	phage portal protein	-
DP45_RS02145	464090..464524	+	435	WP_009969936.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	457191..463286	6095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-100)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12820.92 Da
• Isoelectric Point: 10.3618

>T49295 WP_009972517.1 NZ_CP009585:c459938-459606 [Burkholderia pseudomallei]
MKLDWTVIYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGQQVV
VLHSFVKKTQETPQRELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T49295 NZ_CP009585:c459938-459606 [Burkholderia pseudomallei]
ATGAAACTTGATTGGACGGTCATCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCCGGGATTCTTGC
AGACTACCTCCGGCTGTTGGATCTGATGCAAGAGTTCGGTGCGGATCTACGGATGCCGCATTCACGAGCAATGGGTAACG
GGCTGTTTGAACTGCGCCCCAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCAGCAAGTCGTT
GTGCTGCACTCCTTTGTCAAGAAGACGCAGGAAACGCCGCAACGTGAGTTGCGGATCGCCCAAGCACGTTTGAGGGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11321.89 Da
• Isoelectric Point: 10.2231

>AT49295 WP_009972518.1 NZ_CP009585:c459598-459281 [Burkholderia pseudomallei]
VKRVRAEGFNPVPHTADDTVRLLAGRNVKTAYDALEDEYTALRAILSARREAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKKLVISFA

Nucleotide

• Length: 318 bp

>AT49295 NZ_CP009585:c459598-459281 [Burkholderia pseudomallei]
GTGAAGCGTGTTCGCGCTGAGGGATTCAATCCAGTTCCGCACACAGCGGACGACACGGTGCGTCTGCTCGCCGGTCGCAA
TGTCAAGACGGCGTATGACGCGCTGGAAGACGAATATACGGCGCTGCGCGCGATTCTGTCTGCTCGTCGGGAAGCGGGTC
TGACGCAGGCGCAAATAGCCGAACGTATGGGCACAACCGCGTCAGCGGTTTCAAGGCTTGAGGCATCTTTGTCGAGTGAA
AAGCATTCGCCGTCGTTCGCGACTCTCCGTAAATATGCGGCCGCGTGCGGCAAAAAGCTCGTAATTTCATTTGCTTAG