Detailed information of TA system TA049279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2700143..2700798
• Replicon: chromosome
• Accession: NZ_CP009555
• Organism: Burkholderia oklahomensis C6786

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U4UL57
• Locus tag: BG90_RS36975
• Protein ID: WP_010121214.1
• Coordinates: 2700454..2700798 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BG90_RS36970
• Protein ID: WP_010121216.1
• Coordinates: 2700143..2700457 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG90_RS12130	2696279..2697472	-	1194	WP_010105766.1	pyridoxal phosphate-dependent aminotransferase	-
BG90_RS12135	2697533..2697970	-	438	WP_010105764.1	transcription antitermination factor NusB	-
BG90_RS12140	2697967..2698485	-	519	WP_010105762.1	6,7-dimethyl-8-ribityllumazine synthase	-
BG90_RS12145	2698566..2699702	-	1137	WP_010105760.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	-
BG90_RS36970	2700143..2700457	-	315	WP_010121216.1	transcriptional regulator	Antitoxin
BG90_RS36975	2700454..2700798	-	345	WP_010121214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG90_RS12165	2700896..2701459	+	564	WP_107950779.1	hypothetical protein	-
BG90_RS12170	2701613..2702242	-	630	WP_010105743.1	riboflavin synthase	-
BG90_RS12175	2702257..2703393	-	1137	WP_045568144.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
BG90_RS12180	2703422..2704705	-	1284	WP_010116635.1	glutamate-1-semialdehyde 2,1-aminomutase	-
BG90_RS12185	2705000..2705458	-	459	WP_010116633.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (44-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-90)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13394.28 Da
• Isoelectric Point: 9.1937

>T49279 WP_010121214.1 NZ_CP009555:c2700798-2700454 [Burkholderia oklahomensis C6786]
MEATFIELPTFSRERENYLADDEYAVLQKELLANPEAGDLIKGAGGLRKLRFADKRRQKGKRGGLRVIYYYWVSGYQFWL
FTLYDKDEMADLSSDERKAFAKLLEREVTIRSKQ

Nucleotide

• Length: 345 bp

>T49279 NZ_CP009555:c2700798-2700454 [Burkholderia oklahomensis C6786]
ATGGAAGCGACCTTCATTGAACTGCCGACGTTTTCCCGTGAACGCGAGAACTACCTGGCGGATGACGAGTACGCCGTCCT
GCAGAAAGAGCTGCTGGCCAATCCCGAGGCTGGCGACCTGATCAAGGGAGCCGGCGGTCTGCGGAAGTTGAGGTTCGCCG
ACAAACGCCGCCAGAAAGGCAAACGCGGTGGCCTACGGGTGATCTATTACTACTGGGTCAGCGGTTATCAATTCTGGTTG
TTCACACTGTACGACAAGGATGAAATGGCAGACCTCTCCAGTGACGAGCGCAAAGCCTTCGCAAAGCTACTGGAAAGAGA
GGTCACGATACGGAGCAAGCAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11590.43 Da
• Isoelectric Point: 10.3388

>AT49279 WP_010121216.1 NZ_CP009555:c2700457-2700143 [Burkholderia oklahomensis C6786]
MKKRNLFAELTEGLDALTAEREGKITLRKIKADVPDALEVSPAEIKAVREASHASQAVMARSLRINVRTYQNWEQGKAKP
NAQAATLIRLVAKHPETLQMLETL

Nucleotide

• Length: 315 bp

>AT49279 NZ_CP009555:c2700457-2700143 [Burkholderia oklahomensis C6786]
ATGAAGAAGCGAAATCTGTTTGCTGAATTGACCGAGGGTCTCGATGCCCTTACTGCCGAGCGCGAAGGGAAAATCACGCT
GCGCAAGATCAAGGCCGATGTGCCCGATGCGCTCGAAGTTTCGCCAGCAGAAATCAAGGCCGTGCGTGAAGCCTCTCACG
CGTCGCAAGCCGTCATGGCCCGAAGCCTGCGCATCAATGTGCGTACGTATCAGAATTGGGAACAAGGCAAGGCCAAGCCC
AACGCACAAGCGGCCACGCTGATCCGACTGGTCGCCAAGCACCCGGAAACGCTTCAGATGCTGGAAACGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4UL57