Detailed information of TA system TA049255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 325742..326399
• Replicon: chromosome
• Accession: NZ_CP009549
• Organism: Burkholderia sp. 2002721687

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U4P172
• Locus tag: BW21_RS15000
• Protein ID: WP_009913956.1
• Coordinates: 325742..326074 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BW21_RS34125
• Protein ID: WP_009913954.1
• Coordinates: 326082..326399 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW21_RS14980	321472..322842	+	1371	WP_043281864.1	type II secretory pathway protein	-
BW21_RS14985	322942..323523	+	582	WP_009913959.1	hypothetical protein	-
BW21_RS14990	323968..324747	+	780	WP_043281862.1	hypothetical protein	-
BW21_RS14995	325114..325701	+	588	WP_043282124.1	site-specific integrase	-
BW21_RS15000	325742..326074	+	333	WP_009913956.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BW21_RS34125	326082..326399	+	318	WP_009913954.1	helix-turn-helix domain-containing protein	Antitoxin
BW21_RS34130	326443..327324	+	882	WP_158354520.1	DUF4935 domain-containing protein	-
BW21_RS15020	328317..328550	+	234	WP_009913949.1	hypothetical protein	-
BW21_RS15025	328788..329549	+	762	WP_043281860.1	NYN domain-containing protein	-
BW21_RS15030	329695..330159	+	465	WP_043281857.1	hypothetical protein	-
BW21_RS15035	330156..330440	+	285	WP_006023861.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	310933..343009	32076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-100)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12677.69 Da
• Isoelectric Point: 10.4257

>T49255 WP_009913956.1 NZ_CP009549:325742-326074 [Burkholderia sp. 2002721687]
MTQNWTIVYYNERVKRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGGGLFELRPKGREGIGRVFYCTHVGQRVV
VLHSFVKKTQETPQNELRTARVRLSEVRNG

Nucleotide

• Length: 333 bp

>T49255 NZ_CP009549:325742-326074 [Burkholderia sp. 2002721687]
ATGACGCAAAATTGGACTATTGTCTACTATAACGAGCGCGTCAAGCGCGACGTTTTCGCACTGCCTGCGGGGATCTTGGC
GGATTATCTGCGGCTCCTAGATTTGATGCAGGAGTTTGGTGCTGATCTGCGCATGCCGCATTCGCGGGCAATGGGGGGCG
GATTGTTTGAGTTGCGCCCAAAAGGCAGGGAAGGCATCGGGCGCGTGTTTTACTGCACCCATGTTGGACAGCGGGTCGTG
GTCCTGCATTCGTTTGTGAAGAAAACGCAGGAGACGCCTCAGAATGAGCTGCGAACCGCTCGGGTGCGTTTAAGTGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11340.91 Da
• Isoelectric Point: 8.0784

>AT49255 WP_009913954.1 NZ_CP009549:326082-326399 [Burkholderia sp. 2002721687]
VKRASAEGFNPIQHTVDDTEHLLAKPGVRVAYDALEDEYMALRAILAIRHEAGLTQAQVAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT49255 NZ_CP009549:326082-326399 [Burkholderia sp. 2002721687]
GTGAAGCGTGCTAGTGCTGAAGGCTTCAACCCGATCCAGCACACGGTAGATGATACGGAGCATCTGCTGGCAAAGCCGGG
AGTCAGAGTGGCGTATGATGCTCTGGAAGATGAGTACATGGCTCTGCGGGCCATTTTGGCTATTCGGCATGAGGCCGGAT
TGACGCAAGCTCAAGTGGCAGAGCGCATGGGAACTACGGCGTCAGCGGTTTCGCGACTTGAGGCATCTCTGTCTAGTGAA
AAGCACTCACCCTCCTTTGCGACGCTGCGCAAGTATGCTGCTGCGTGTGGCAAGAGATTGGTGATTTCGTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7U4P172