Detailed information of TA system TA049162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1589..2229
• Replicon: plasmid pPCP1
• Accession: NZ_CP009489
• Organism: Yersinia pestis strain PBM19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CH59_RS00020
• Protein ID: WP_002225538.1
• Coordinates: 1888..2229 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: CH59_RS00015
• Protein ID: WP_002213446.1
• Coordinates: 1589..1888 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH59_RS00010	464..1402	+	939	WP_002218234.1	omptin family plasminogen activator Pla	-
CH59_RS00015	1589..1888	-	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
CH59_RS00020	1888..2229	-	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH59_RS00030	3496..4518	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
CH59_RS00035	4518..5297	+	780	WP_001297096.1	IS21-like element IS100kyp family helper ATPase IstB	-
CH59_RS00040	6334..6528	+	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla	1..9610	9610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T49162 WP_002225538.1 NZ_CP009489:c2229-1888 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T49162 NZ_CP009489:c2229-1888 [Yersinia pestis]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT49162 WP_002213446.1 NZ_CP009489:c1888-1589 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT49162 NZ_CP009489:c1888-1589 [Yersinia pestis]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016