Detailed information of TA system TA049156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1393595..1394188
• Replicon: chromosome
• Accession: NZ_CP009486
• Organism: Burkholderia ubonensis MSMB22

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A124NDB4
• Locus tag: BW23_RS06130
• Protein ID: WP_042588736.1
• Coordinates: 1393910..1394188 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BW23_RS06125
• Protein ID: WP_045564533.1
• Coordinates: 1393595..1393897 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW23_RS06100	1388843..1390114	+	1272	WP_045564529.1	Rieske 2Fe-2S domain-containing protein	-
BW23_RS06105	1390129..1390605	+	477	WP_042587286.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
BW23_RS06110	1390625..1390951	+	327	WP_045564530.1	non-heme iron oxygenase ferredoxin subunit	-
BW23_RS06115	1390941..1392161	+	1221	WP_045564531.1	FAD-dependent oxidoreductase	-
BW23_RS06120	1392252..1393538	+	1287	WP_045564532.1	DUF445 family protein	-
BW23_RS06125	1393595..1393897	-	303	WP_045564533.1	HigA family addiction module antidote protein	Antitoxin
BW23_RS06130	1393910..1394188	-	279	WP_042588736.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BW23_RS06135	1394570..1394869	-	300	WP_010089041.1	putative addiction module antidote protein	-
BW23_RS06140	1394873..1395127	-	255	Protein_1265	type II toxin-antitoxin system RelE/ParE family toxin	-
BW23_RS06145	1395541..1397181	+	1641	WP_010089043.1	acetolactate synthase large subunit	-
BW23_RS06150	1397211..1398644	+	1434	WP_045564534.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10684.07 Da
• Isoelectric Point: 7.8922

>T49156 WP_042588736.1 NZ_CP009486:c1394188-1393910 [Burkholderia ubonensis MSMB22]
MIRSFRCKDTQSLFAGESPKRFRTIESVATRKLEMVAAAKELRDLRSPPGNRLEALHGNRDGQHSIRINEQWRICFEWTD
DGPANVEILDYH

Nucleotide

• Length: 279 bp

>T49156 NZ_CP009486:c1394188-1393910 [Burkholderia ubonensis MSMB22]
ATGATCAGATCGTTCCGATGCAAGGATACCCAGTCGCTTTTCGCCGGTGAATCACCCAAACGCTTCCGTACGATCGAATC
CGTTGCTACGCGGAAACTGGAAATGGTCGCTGCTGCGAAAGAACTGCGCGACTTGCGGTCTCCGCCCGGCAACCGGCTCG
AAGCGCTTCACGGAAATCGGGATGGCCAGCACAGCATCCGTATCAACGAGCAATGGCGGATCTGCTTCGAATGGACCGAC
GACGGCCCCGCCAACGTCGAGATCCTTGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11615.36 Da
• Isoelectric Point: 8.5562

>AT49156 WP_045564533.1 NZ_CP009486:c1393897-1393595 [Burkholderia ubonensis MSMB22]
MLKNGMRPIHPGEILREEFLTPLEMSVNALALALNVPATRLHEIVKERRGVTADTAYRLARYFDTSPEFWLNLQATYDLK
TLPTRNEIDRKVEPREHTRA

Nucleotide

• Length: 303 bp

>AT49156 NZ_CP009486:c1393897-1393595 [Burkholderia ubonensis MSMB22]
ATGCTCAAGAATGGAATGCGTCCGATCCACCCGGGCGAAATCCTGCGAGAAGAATTCCTGACGCCGCTGGAAATGAGTGT
CAACGCGCTTGCACTCGCGCTGAACGTACCGGCTACCCGCCTGCATGAAATCGTCAAGGAACGGCGCGGGGTCACGGCCG
ATACCGCTTACCGGCTCGCACGCTACTTCGACACGTCACCCGAGTTCTGGCTGAACCTGCAAGCCACATACGACCTCAAA
ACGCTGCCGACGCGCAATGAGATCGACCGGAAGGTTGAACCGCGCGAACACACCCGCGCATAG

Structures

Toxin

• AlphaFold DB: A0A124NDB4