Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3491001..3491598 Replicon chromosome
Accession NZ_CP009461
Organism Klebsiella pneumoniae strain XH209

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LQ47_RS17310 Protein ID WP_004142563.1
Coordinates 3491281..3491598 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LQ47_RS17305 Protein ID WP_004142561.1
Coordinates 3491001..3491288 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ47_RS17275 3487081..3487329 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LQ47_RS17280 3487347..3487688 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LQ47_RS17285 3487719..3488834 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LQ47_RS17290 3489014..3489595 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LQ47_RS17295 3489595..3489963 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LQ47_RS17300 3490083..3490736 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LQ47_RS17305 3491001..3491288 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LQ47_RS17310 3491281..3491598 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LQ47_RS17315 3491783..3492826 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
LQ47_RS17320 3493495..3494361 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LQ47_RS17325 3494470..3495897 + 1428 WP_023284820.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T49125 WP_004142563.1 NZ_CP009461:c3491598-3491281 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T49125 NZ_CP009461:c3491598-3491281 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT49125 WP_004142561.1 NZ_CP009461:c3491288-3491001 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT49125 NZ_CP009461:c3491288-3491001 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References