Detailed information of TA system TA049029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4393400..4394061
• Replicon: chromosome
• Accession: NZ_CP009451
• Organism: Cedecea neteri strain SSMD04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A089Q3T4
• Locus tag: JT31_RS20270
• Protein ID: WP_038481388.1
• Coordinates: 4393400..4393774 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JT31_RS20275
• Protein ID: WP_038481391.1
• Coordinates: 4393759..4394061 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT31_RS20250	4388822..4389880	+	1059	WP_038481376.1	FUSC family protein	-
JT31_RS20255	4390055..4390390	+	336	WP_038481379.1	DUF496 family protein	-
JT31_RS20260	4390582..4390890	-	309	WP_038481382.1	helix-turn-helix domain-containing protein	-
JT31_RS20265	4391083..4393185	+	2103	WP_038481385.1	elongation factor G	-
JT31_RS20270	4393400..4393774	+	375	WP_038481388.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JT31_RS20275	4393759..4394061	+	303	WP_038481391.1	XRE family transcriptional regulator	Antitoxin
JT31_RS20280	4394108..4394482	-	375	WP_038481395.1	DUF4440 domain-containing protein	-
JT31_RS20285	4394486..4395862	-	1377	WP_038481398.1	MFS transporter	-
JT31_RS20290	4395973..4396872	+	900	WP_038481401.1	LysR family transcriptional regulator	-
JT31_RS20295	4396890..4398416	-	1527	WP_052049030.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14275.56 Da
• Isoelectric Point: 8.7204

>T49029 WP_038481388.1 NZ_CP009451:4393400-4393774 [Cedecea neteri]
MWSIKMTDTFDAWFTASDDAIKASVIASMVLLKSRGPLLSRPYADTVRGSQYANMKELRVQCRGEPLRLFFAFDIRRCAI
LLCAGNKAGNEKRFYDVMIPRADKEFAAHLKQLTEKEQIEWVEH

Nucleotide

• Length: 375 bp

>T49029 NZ_CP009451:4393400-4393774 [Cedecea neteri]
ATGTGGTCGATAAAAATGACGGATACGTTTGATGCCTGGTTTACGGCATCGGATGATGCAATCAAAGCCAGCGTCATCGC
ATCAATGGTGTTGCTGAAGAGCAGAGGACCTTTACTAAGCAGGCCTTACGCAGACACGGTGAGAGGTTCGCAATACGCCA
ACATGAAAGAGCTGCGCGTTCAGTGCAGAGGCGAACCATTAAGGCTATTTTTCGCTTTTGATATAAGACGTTGCGCCATC
CTGCTGTGTGCTGGCAATAAGGCCGGAAACGAGAAACGATTTTATGATGTCATGATTCCTCGGGCCGATAAGGAATTTGC
AGCTCACCTTAAACAGTTAACGGAAAAGGAGCAGATAGAATGGGTCGAACACTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11097.93 Da
• Isoelectric Point: 7.2054

>AT49029 WP_038481391.1 NZ_CP009451:4393759-4394061 [Cedecea neteri]
MGRTLEELLATEKPEVVAAAQAMADEMLLNIHLAELRDRVRKTQNEMAQKLGVKQPTVAEMEKKGRDIKLSSLKRYVEAA
GGKVQLDVELPDGSHFKIAL

Nucleotide

• Length: 303 bp

>AT49029 NZ_CP009451:4393759-4394061 [Cedecea neteri]
ATGGGTCGAACACTAGAAGAGCTACTTGCCACGGAAAAACCAGAAGTTGTCGCCGCCGCGCAGGCCATGGCCGATGAAAT
GCTGCTGAATATCCATCTGGCTGAGCTACGCGATCGGGTAAGGAAAACGCAGAACGAGATGGCGCAAAAGCTGGGCGTGA
AGCAGCCGACCGTTGCCGAAATGGAAAAAAAAGGCCGTGATATCAAGCTATCCTCGCTCAAACGCTATGTCGAAGCGGCG
GGCGGCAAGGTTCAGCTGGATGTTGAGCTACCGGACGGCTCTCACTTTAAAATTGCGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A089Q3T4