Detailed information of TA system TA049022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2888843..2889561
• Replicon: chromosome
• Accession: NZ_CP009451
• Organism: Cedecea neteri strain SSMD04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A089Q574
• Locus tag: JT31_RS13305
• Protein ID: WP_038477862.1
• Coordinates: 2889250..2889561 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JT31_RS13300
• Protein ID: WP_038477859.1
• Coordinates: 2888843..2889253 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT31_RS13275	2885293..2885934	+	642	WP_038483112.1	FMN-binding negative transcriptional regulator	-
JT31_RS13280	2885941..2886990	-	1050	WP_038477852.1	AI-2E family transporter	-
JT31_RS13285	2887121..2887678	-	558	WP_038477855.1	DcrB family lipoprotein	-
JT31_RS13290	2887751..2888416	-	666	WP_016538599.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
JT31_RS13295	2888591..2888836	+	246	WP_008458604.1	sulfurtransferase TusA	-
JT31_RS13300	2888843..2889253	-	411	WP_038477859.1	helix-turn-helix domain-containing protein	Antitoxin
JT31_RS13305	2889250..2889561	-	312	WP_038477862.1	type II toxin-antitoxin system HigB family toxin	Toxin
JT31_RS13310	2889704..2891917	-	2214	WP_038477865.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
JT31_RS13315	2892102..2893148	+	1047	WP_038477868.1	LacI family transcriptional regulator	-
JT31_RS13320	2893312..2894382	+	1071	WP_038477871.1	SIS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12406.59 Da
• Isoelectric Point: 10.6835

>T49022 WP_038477862.1 NZ_CP009451:c2889561-2889250 [Cedecea neteri]
MHLITLKTLIEAGQRFPRHRQELQILGKVLEKGFFPTPESLKRIYPSLDNFKYLDKHYVINIAGNELRLIALIFFTSQKF
YIRHIMTHSEYMRFTETHRGKKR

Nucleotide

• Length: 312 bp

>T49022 NZ_CP009451:c2889561-2889250 [Cedecea neteri]
ATGCATCTGATTACGCTGAAGACGCTTATAGAAGCGGGTCAAAGATTTCCCAGACATAGGCAGGAACTGCAAATTCTCGG
CAAAGTGCTGGAAAAAGGCTTCTTTCCAACGCCGGAATCACTTAAGCGGATTTATCCTTCACTGGATAACTTTAAGTACC
TCGATAAACATTACGTCATCAATATCGCCGGCAATGAACTCAGGCTGATTGCGCTTATCTTTTTTACCAGCCAGAAGTTC
TACATCCGCCATATCATGACGCACAGCGAATACATGCGGTTTACTGAAACACACCGGGGAAAGAAGAGATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15192.57 Da
• Isoelectric Point: 6.2387

>AT49022 WP_038477859.1 NZ_CP009451:c2889253-2888843 [Cedecea neteri]
MIADALKAAEALKTAVPLLGGSTREKDYLAALELVEYLLLNDPENPLVEIVSSKISAWENAQPEVQTFRAEMQAIPAGIA
MLKTLMEHHQLKQSDFQQEIGSKSMVSRILSGERQLTTEHIRRLSRRFGISPALFF

Nucleotide

• Length: 411 bp

>AT49022 NZ_CP009451:c2889253-2888843 [Cedecea neteri]
ATGATTGCTGATGCGCTTAAAGCCGCCGAAGCATTGAAAACGGCGGTTCCACTGCTGGGTGGCAGCACCAGAGAAAAAGA
TTATCTGGCGGCGCTGGAGCTGGTCGAATACCTGCTGCTCAATGACCCAGAAAACCCACTGGTTGAGATTGTCAGCAGCA
AAATCTCTGCCTGGGAAAACGCACAGCCTGAAGTACAGACGTTCCGAGCGGAAATGCAGGCAATCCCGGCCGGGATAGCC
ATGCTGAAAACCCTGATGGAGCATCACCAATTAAAACAGAGCGATTTTCAGCAAGAGATTGGCAGCAAGTCGATGGTATC
CCGCATTCTCAGCGGCGAACGACAGCTAACCACCGAACACATCCGCCGGCTTTCCCGGCGCTTTGGCATCTCACCTGCCC
TCTTTTTCTGA

Structures

Toxin

• AlphaFold DB: A0A089Q574