Detailed information of TA system TA049008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2813060..2813728
• Replicon: chromosome
• Accession: NZ_CP009450
• Organism: Pluralibacter gergoviae strain FB2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806NUW5
• Locus tag: LG71_RS13080
• Protein ID: WP_043083051.1
• Coordinates: 2813375..2813728 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LG71_RS13075
• Protein ID: WP_043083049.1
• Coordinates: 2813060..2813371 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG71_RS13050	2808209..2808766	-	558	WP_043083043.1	HTH-type transcriptional regulator PuuR	-
LG71_RS13055	2808792..2809556	-	765	WP_043086483.1	gamma-glutamyl-gamma-aminobutyrate hydrolase	-
LG71_RS13060	2809769..2811187	+	1419	WP_043083044.1	glutamine synthetase family protein	-
LG71_RS13065	2811454..2812842	+	1389	WP_043083047.1	APC family permease	-
LG71_RS13075	2813060..2813371	-	312	WP_043083049.1	XRE family transcriptional regulator	Antitoxin
LG71_RS13080	2813375..2813728	-	354	WP_043083051.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG71_RS13085	2813910..2814389	+	480	WP_043083052.1	GNAT family N-acetyltransferase	-
LG71_RS13090	2814427..2815881	-	1455	WP_043083053.1	AMP nucleosidase	-
LG71_RS13095	2815986..2816312	-	327	WP_052097992.1	hypothetical protein	-
LG71_RS13100	2816673..2817860	+	1188	Protein_2638	GTP-binding protein	-
LG71_RS13105	2817863..2818102	-	240	WP_043086484.1	hypothetical protein	-
LG71_RS13110	2818210..2818629	+	420	WP_043083055.1	RNA polymerase-binding protein DksA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13753.66 Da
• Isoelectric Point: 8.0240

>T49008 WP_043083051.1 NZ_CP009450:c2813728-2813375 [Pluralibacter gergoviae]
VWNIQITETYKAWHQALNAKDQARVLAMLGLLEKEGPRLGRPYADTIRGSRFSNMKELRIQHRGEPLRIFYVFSPEREGI
LLCAGNKSGDDKRFYDVMIPLADEEYTEYLNHFYQSR

Nucleotide

• Length: 354 bp

>T49008 NZ_CP009450:c2813728-2813375 [Pluralibacter gergoviae]
GTGTGGAACATTCAGATAACAGAAACCTATAAAGCCTGGCATCAGGCGCTGAACGCTAAAGACCAGGCGCGGGTGCTTGC
CATGCTCGGCCTGCTGGAAAAGGAAGGTCCCCGGCTCGGCAGGCCGTATGCCGATACGATAAGGGGCTCCCGCTTCAGCA
ATATGAAAGAGCTCAGGATCCAGCACCGGGGCGAGCCGCTGCGCATTTTTTACGTCTTTTCGCCTGAGCGTGAAGGCATC
CTGCTGTGTGCGGGCAATAAATCCGGCGATGATAAGCGTTTTTATGACGTGATGATCCCGCTGGCCGATGAAGAATACAC
TGAATATCTCAACCATTTTTATCAGTCGAGGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11551.30 Da
• Isoelectric Point: 4.8950

>AT49008 WP_043083049.1 NZ_CP009450:c2813371-2813060 [Pluralibacter gergoviae]
MVTTLNQLRAETAPEILAEADALYQELLLEYNLAKLREMVNKTQQEMADSLGIKQPTLAGMEKKGNDIKLSSLKRYVEAA
GCRLNLDIELPDGTHCAFRLSER

Nucleotide

• Length: 312 bp

>AT49008 NZ_CP009450:c2813371-2813060 [Pluralibacter gergoviae]
ATGGTAACAACACTGAACCAGCTGCGAGCGGAAACAGCGCCGGAAATTCTCGCCGAAGCCGACGCCCTCTATCAGGAGCT
GCTGCTGGAGTACAACCTGGCGAAGCTGCGTGAGATGGTCAATAAGACCCAGCAGGAGATGGCCGACAGTCTGGGCATTA
AGCAGCCGACGCTTGCCGGCATGGAGAAAAAGGGCAACGACATCAAGCTCTCTTCCCTCAAGCGCTACGTAGAGGCGGCG
GGCTGCCGGCTGAATCTGGATATCGAGCTGCCGGACGGCACGCACTGCGCGTTCCGGCTTTCGGAACGCTAG

Structures

Toxin

• AlphaFold DB: A0A806NUW5