Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3556023..3556693 Replicon chromosome
Accession NZ_CP009427
Organism Mycobacterium tuberculosis strain 96121

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag HR37_RS16870 Protein ID WP_003899954.1
Coordinates 3556023..3556367 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag HR37_RS16875 Protein ID WP_003899955.1
Coordinates 3556364..3556693 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR37_RS16840 3551096..3551956 + 861 WP_003416628.1 alpha/beta hydrolase -
HR37_RS16845 3551931..3552446 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
HR37_RS16850 3552686..3552979 + 294 WP_003416635.1 hypothetical protein -
HR37_RS16855 3553267..3554556 + 1290 WP_003416640.1 ATP-binding protein -
HR37_RS16860 3554903..3555337 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
HR37_RS16865 3555340..3555792 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HR37_RS16870 3556023..3556367 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR37_RS16875 3556364..3556693 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
HR37_RS16885 3556770..3558031 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
HR37_RS16890 3558316..3558663 + 348 WP_003899956.1 DUF2384 domain-containing protein -
HR37_RS16895 3558660..3559280 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
HR37_RS16900 3559440..3560705 - 1266 Protein_3301 hypothetical protein -
HR37_RS16905 3560873..3561082 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T48986 WP_003899954.1 NZ_CP009427:3556023-3556367 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T48986 NZ_CP009427:3556023-3556367 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT48986 WP_003899955.1 NZ_CP009427:3556364-3556693 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT48986 NZ_CP009427:3556364-3556693 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References