Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2953871..2954519 Replicon chromosome
Accession NZ_CP009426
Organism Mycobacterium tuberculosis strain 96075

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag LJ70_RS14000 Protein ID WP_003899414.1
Coordinates 2953871..2954194 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag LJ70_RS14005 Protein ID WP_003899415.1
Coordinates 2954274..2954519 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ70_RS13970 2949194..2950192 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
LJ70_RS13975 2950206..2950670 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
LJ70_RS13980 2950658..2950909 + 252 WP_003908028.1 hypothetical protein -
LJ70_RS13985 2951080..2952519 - 1440 WP_003901443.1 phage major capsid protein -
LJ70_RS13990 2952527..2953060 - 534 WP_003899412.1 HK97 family phage prohead protease -
LJ70_RS21580 2953213..2953704 - 492 WP_003900541.1 phage terminase small subunit P27 family -
LJ70_RS14000 2953871..2954194 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
LJ70_RS14005 2954274..2954519 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
LJ70_RS14010 2954516..2955943 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
LJ70_RS14015 2955945..2956337 - 393 WP_003899417.1 DUF2742 domain-containing protein -
LJ70_RS14020 2956334..2956594 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
LJ70_RS14025 2956611..2956973 - 363 WP_003900543.1 hypothetical protein -
LJ70_RS14030 2956976..2958103 - 1128 WP_003899420.1 site-specific integrase -
LJ70_RS14035 2958248..2958475 - 228 WP_003899421.1 hypothetical protein -
LJ70_RS21585 2958472..2958861 - 390 WP_003899422.1 hypothetical protein -
LJ70_RS14040 2958767..2959039 + 273 WP_003900544.1 hypothetical protein -
LJ70_RS14045 2959138..2959371 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2949194..2958103 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T48918 WP_003899414.1 NZ_CP009426:c2954194-2953871 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T48918 NZ_CP009426:c2954194-2953871 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGATAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT48918 WP_003899415.1 NZ_CP009426:c2954519-2954274 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT48918 NZ_CP009426:c2954519-2954274 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References