Detailed information of TA system TA048852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1913106..1913703
• Replicon: chromosome
• Accession: NZ_CP009422
• Organism: Neisseria meningitidis strain M10208

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: LD07_RS09995
• Protein ID: WP_002242826.1
• Coordinates: 1913425..1913703 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LD07_RS09990
• Protein ID: WP_002221020.1
• Coordinates: 1913106..1913417 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD07_RS14455	1908975..1909139	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
LD07_RS09975	1909139..1909750	+	612	WP_042744707.1	baseplate J/gp47 family protein	-
LD07_RS09980	1909747..1910307	+	561	WP_002213590.1	YmfQ family protein	-
LD07_RS09985	1910318..1912291	+	1974	WP_002248050.1	pyocin knob domain-containing protein	-
LD07_RS09990	1913106..1913417	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
LD07_RS09995	1913425..1913703	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LD07_RS10000	1913788..1914393	+	606	WP_002219301.1	hypothetical protein	-
LD07_RS10005	1914372..1914668	+	297	WP_002221017.1	hypothetical protein	-
LD07_RS10010	1914665..1914874	+	210	WP_002222487.1	hypothetical protein	-
LD07_RS10015	1914984..1916127	-	1144	Protein_1835	ABC transporter ATP-binding protein/permease	-
LD07_RS10030	1916416..1917063	-	648	WP_002217218.1	DUF799 domain-containing protein	-
LD07_RS10035	1917060..1917428	-	369	WP_002234874.1	DUF4810 domain-containing protein	-
LD07_RS10040	1917438..1918109	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1901093..1914668	13575
	-	flank	IS/Tn	-	-	1907945..1908910	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T48852 WP_002242826.1 NZ_CP009422:c1913703-1913425 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T48852 NZ_CP009422:c1913703-1913425 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT48852 WP_002221020.1 NZ_CP009422:c1913417-1913106 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT48852 NZ_CP009422:c1913417-1913106 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6