Detailed information of TA system TA048848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 63795..64392
• Replicon: chromosome
• Accession: NZ_CP009418
• Organism: Neisseria meningitidis strain NM3686

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: LA50_RS00425
• Protein ID: WP_002242826.1
• Coordinates: 63795..64073 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LA50_RS00430
• Protein ID: WP_002221020.1
• Coordinates: 64081..64392 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA50_RS00380	59389..60060	+	672	WP_002217215.1	CsgG/HfaB family protein	-
LA50_RS00385	60070..60438	+	369	WP_002234874.1	DUF4810 domain-containing protein	-
LA50_RS00390	60435..61082	+	648	WP_002217218.1	DUF799 domain-containing protein	-
LA50_RS00405	61371..62514	+	1144	Protein_68	ABC transporter ATP-binding protein/permease	-
LA50_RS00410	62624..62833	-	210	WP_002222487.1	hypothetical protein	-
LA50_RS00415	62830..63126	-	297	WP_002221017.1	hypothetical protein	-
LA50_RS00420	63105..63710	-	606	WP_002219301.1	hypothetical protein	-
LA50_RS00425	63795..64073	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA50_RS00430	64081..64392	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
LA50_RS00435	65158..67131	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
LA50_RS00440	67142..67702	-	561	WP_002213590.1	YmfQ family protein	-
LA50_RS00445	67699..68310	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
LA50_RS13540	68310..68474	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	62830..76356	13526
	-	flank	IS/Tn	-	-	68539..69504	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T48848 WP_002242826.1 NZ_CP009418:63795-64073 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T48848 NZ_CP009418:63795-64073 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT48848 WP_002221020.1 NZ_CP009418:64081-64392 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT48848 NZ_CP009418:64081-64392 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6