Detailed information of TA system TA048698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3161210..3161903
• Replicon: chromosome
• Accession: NZ_CP009273
• Organism: Escherichia coli BW25113

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: BW25113_RS15685
• Protein ID: WP_000415584.1
• Coordinates: 3161607..3161903 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: BW25113_RS15680
• Protein ID: WP_000650107.1
• Coordinates: 3161210..3161605 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW25113_RS15670 (BW25113_3019)	3157074..3159332	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
BW25113_RS15675 (BW25113_3020)	3159470..3161077	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
BW25113_RS15680 (BW25113_3021)	3161210..3161605	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
BW25113_RS15685 (BW25113_3022)	3161607..3161903	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
BW25113_RS15690 (BW25113_3023)	3162108..3162590	-	483	WP_000183505.1	transcriptional regulator	-
BW25113_RS15695 (BW25113_3024)	3162643..3163035	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BW25113_RS15700 (BW25113_3025)	3163187..3163846	+	660	WP_001221495.1	two-component system response regulator QseB	-
BW25113_RS15705 (BW25113_3026)	3163843..3165192	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
BW25113_RS15710 (BW25113_3027)	3165238..3165570	-	333	WP_000917684.1	DUF2645 family protein	-
BW25113_RS15715 (BW25113_3028)	3165889..3166470	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BW25113_RS15720 (BW25113_3029)	3166501..3166815	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T48698 WP_000415584.1 NZ_CP009273:c3161903-3161607 [Escherichia coli BW25113]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T48698 NZ_CP009273:c3161903-3161607 [Escherichia coli BW25113]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT48698 WP_000650107.1 NZ_CP009273:c3161605-3161210 [Escherichia coli BW25113]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT48698 NZ_CP009273:c3161605-3161210 [Escherichia coli BW25113]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65