Detailed information of TA system TA048641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3498220..3498890
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: BCGR_RS16460
• Protein ID: WP_003899954.1
• Coordinates: 3498220..3498564 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: BCGR_RS16465
• Protein ID: WP_003899955.1
• Coordinates: 3498561..3498890 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS16430	3493293..3494153	+	861	WP_003416628.1	alpha/beta hydrolase	-
BCGR_RS16435	3494128..3494643	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
BCGR_RS16440	3494883..3495176	+	294	WP_003416635.1	hypothetical protein	-
BCGR_RS16445	3495464..3496753	+	1290	WP_003416640.1	ATP-binding protein	-
BCGR_RS16450	3497100..3497534	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
BCGR_RS16455	3497537..3497989	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BCGR_RS16460	3498220..3498564	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCGR_RS16465	3498561..3498890	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
BCGR_RS16470	3499428..3499775	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
BCGR_RS16475	3499772..3500392	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
BCGR_RS16480	3500552..3501817	-	1266	WP_003909837.1	hypothetical protein	-
BCGR_RS16485	3501985..3502194	+	210	WP_003416778.1	hypothetical protein	-
BCGR_RS16495	3502441..3503475	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T48641 WP_003899954.1 NZ_CP009243:3498220-3498564 [Mycobacterium tuberculosis variant bovis BCG]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T48641 NZ_CP009243:3498220-3498564 [Mycobacterium tuberculosis variant bovis BCG]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT48641 WP_003899955.1 NZ_CP009243:3498561-3498890 [Mycobacterium tuberculosis variant bovis BCG]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT48641 NZ_CP009243:3498561-3498890 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6