Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2185873..2186741 Replicon chromosome
Accession NZ_CP009243
Organism Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag BCGR_RS10145 Protein ID WP_010886136.1
Coordinates 2185873..2186250 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag BCGR_RS10150 Protein ID WP_003409886.1
Coordinates 2186292..2186741 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCGR_RS10095 2181662..2182114 - 453 WP_010950636.1 lipoprotein -
BCGR_RS10100 2182178..2182579 + 402 WP_003409869.1 hypothetical protein -
BCGR_RS10105 2182572..2182754 - 183 WP_003409870.1 hypothetical protein -
BCGR_RS10110 2182868..2183218 - 351 WP_003409871.1 hypothetical protein -
BCGR_RS10115 2183229..2184131 - 903 WP_003409874.1 hypothetical protein -
BCGR_RS10120 2184152..2184343 - 192 WP_003409876.1 hypothetical protein -
BCGR_RS10125 2184344..2184640 - 297 WP_003409877.1 hypothetical protein -
BCGR_RS10130 2184880..2185095 + 216 WP_003409878.1 antitoxin -
BCGR_RS10135 2185092..2185403 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
BCGR_RS22115 2185377..2185898 - 522 WP_010950637.1 hypothetical protein -
BCGR_RS10145 2185873..2186250 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
BCGR_RS10150 2186292..2186741 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
BCGR_RS10155 2186738..2187283 + 546 WP_003409891.1 SecB-like chaperone -
BCGR_RS10160 2187172..2187786 - 615 WP_003901296.1 hypothetical protein -
BCGR_RS10165 2187835..2188131 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
BCGR_RS10170 2188128..2188379 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
BCGR_RS10175 2188366..2188860 + 495 WP_003899099.1 hypothetical protein -
BCGR_RS10180 2189020..2189427 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
BCGR_RS10185 2189431..2189703 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BCGR_RS10190 2189736..2190956 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T48618 WP_010886136.1 NZ_CP009243:2185873-2186250 [Mycobacterium tuberculosis variant bovis BCG]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T48618 NZ_CP009243:2185873-2186250 [Mycobacterium tuberculosis variant bovis BCG]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT48618 WP_003409886.1 NZ_CP009243:2186292-2186741 [Mycobacterium tuberculosis variant bovis BCG]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT48618 NZ_CP009243:2186292-2186741 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References