Detailed information of TA system TA048578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 305933..306494
• Replicon: chromosome
• Accession: NZ_CP009237
• Organism: Glaesserella parasuis strain KL0318

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: JT17_RS01615
• Protein ID: WP_005712614.1
• Coordinates: 305933..306211 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: JT17_RS01620
• Protein ID: WP_005712616.1
• Coordinates: 306222..306494 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT17_RS01600	301725..303053	-	1329	WP_044009532.1	exodeoxyribonuclease VII large subunit	-
JT17_RS01605	303167..304429	-	1263	WP_005712610.1	serine hydroxymethyltransferase	-
JT17_RS01610	304776..305786	-	1011	WP_044008995.1	ornithine carbamoyltransferase	-
JT17_RS01615	305933..306211	+	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JT17_RS01620	306222..306494	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
JT17_RS01625	306558..307778	+	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
JT17_RS01630	307788..308096	+	309	Protein_325	DUF853 family protein	-
JT17_RS01635	308168..309382	-	1215	WP_021114868.1	ATP-binding protein	-
JT17_RS01640	309589..310128	-	540	WP_005714441.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T48578 WP_005712614.1 NZ_CP009237:305933-306211 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T48578 NZ_CP009237:305933-306211 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACCGCAGGTATTCAAGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATTTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT48578 WP_005712616.1 NZ_CP009237:306222-306494 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT48578 NZ_CP009237:306222-306494 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTCTCTCGTATTTTAAATGGCAAAGCCGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: A0A143CE91