Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2012238..2012835 Replicon chromosome
Accession NZ_CP009208
Organism Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag VK055_RS09965 Protein ID WP_004142563.1
Coordinates 2012518..2012835 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag VK055_RS09960 Protein ID WP_004142561.1
Coordinates 2012238..2012525 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK055_RS09930 2007508..2008623 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
VK055_RS09935 2008803..2009387 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
VK055_RS09940 2009384..2009752 + 369 WP_002893024.1 MmcQ/YjbR family DNA-binding protein -
VK055_RS09945 2009872..2010525 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
VK055_RS28025 2010711..2012074 + 1364 WP_087661600.1 IS3-like element ISKpn1 family transposase -
VK055_RS09960 2012238..2012525 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
VK055_RS09965 2012518..2012835 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VK055_RS09970 2013020..2014063 - 1044 WP_038431985.1 DUF2157 domain-containing protein -
VK055_RS09975 2014733..2015599 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
VK055_RS09980 2015708..2017135 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T48547 WP_004142563.1 NZ_CP009208:c2012835-2012518 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T48547 NZ_CP009208:c2012835-2012518 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT48547 WP_004142561.1 NZ_CP009208:c2012525-2012238 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT48547 NZ_CP009208:c2012525-2012238 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References