Detailed information of TA system TA048531

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65392..65813
• Replicon: plasmid p1303_109
• Accession: NZ_CP009167
• Organism: Escherichia coli 1303

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: EC1303_RS24650
• Protein ID: WP_001302184.1
• Coordinates: 65655..65813 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65392..65590 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC1303_RS24620	61323..61862	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
EC1303_RS24625	61919..62152	+	234	WP_000005990.1	DUF905 family protein	-
EC1303_RS24630	62218..64176	+	1959	WP_000117172.1	ParB/RepB/Spo0J family partition protein	-
EC1303_RS24635	64231..64664	+	434	Protein_53	conjugation system SOS inhibitor PsiB	-
EC1303_RS24640	64661..65380	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	65392..65590	+	199	NuclAT_0	-	Antitoxin
-	65392..65590	+	199	NuclAT_0	-	Antitoxin
-	65392..65590	+	199	NuclAT_0	-	Antitoxin
-	65392..65590	+	199	NuclAT_0	-	Antitoxin
EC1303_RS24650	65655..65813	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
EC1303_RS29360	66051..66410	-	360	Protein_56	hypothetical protein	-
EC1303_RS24665	66711..66998	+	288	WP_000107542.1	hypothetical protein	-
EC1303_RS24670	67117..67938	+	822	WP_001234474.1	DUF945 domain-containing protein	-
EC1303_RS24675	68233..68823	-	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
EC1303_RS24680	69157..69540	+	384	WP_000124981.1	relaxosome protein TraM	-
EC1303_RS24685	69730..70416	+	687	WP_000332492.1	PAS domain-containing protein	-
EC1303_RS24690	70510..70737	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108501	108501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T48531 WP_001302184.1 NZ_CP009167:65655-65813 [Escherichia coli 1303]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T48531 NZ_CP009167:65655-65813 [Escherichia coli 1303]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT48531 NZ_CP009167:65392-65590 [Escherichia coli 1303]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin