Detailed information of TA system TA048507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 298255..298949
• Replicon: chromosome
• Accession: NZ_CP009166
• Organism: Escherichia coli 1303

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: EC1303_RS01415
• Protein ID: WP_001263493.1
• Coordinates: 298551..298949 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: EC1303_RS01410
• Protein ID: WP_000554757.1
• Coordinates: 298255..298548 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC1303_RS01385	294072..294539	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
EC1303_RS01390	294559..295275	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
EC1303_RS01395	295288..296151	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
EC1303_RS01400	296154..297077	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
EC1303_RS01405	297148..298203	+	1056	WP_001226164.1	DNA polymerase IV	-
EC1303_RS01410	298255..298548	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EC1303_RS01415	298551..298949	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EC1303_RS01420	298959..299411	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
EC1303_RS01425	299657..299860	+	204	Protein_275	RNA ligase RtcB family protein	-
EC1303_RS01430	299859..300380	+	522	Protein_276	peptide chain release factor H	-
EC1303_RS01435	300437..301894	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
EC1303_RS01440	302155..302613	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
EC1303_RS01445	302705..303949	+	1245	WP_000189541.1	esterase FrsA	-
-	303209..303289	+	81	NuclAT_10	-	-
-	303209..303289	+	81	NuclAT_10	-	-
-	303209..303289	+	81	NuclAT_10	-	-
-	303209..303289	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T48507 WP_001263493.1 NZ_CP009166:298551-298949 [Escherichia coli 1303]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T48507 NZ_CP009166:298551-298949 [Escherichia coli 1303]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT48507 WP_000554757.1 NZ_CP009166:298255-298548 [Escherichia coli 1303]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT48507 NZ_CP009166:298255-298548 [Escherichia coli 1303]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6