Detailed information of TA system TA048491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2046038..2046692
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0G6E5
• Locus tag: ASU1_RS09440
• Protein ID: WP_015674148.1
• Coordinates: 2046330..2046692 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASU1_RS09435
• Protein ID: WP_015674147.1
• Coordinates: 2046038..2046346 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS09410	2041183..2041749	-	567	WP_015674143.1	septal ring lytic transglycosylase RlpA family protein	-
ASU1_RS09415	2041924..2043048	-	1125	WP_015674144.1	rod shape-determining protein RodA	-
ASU1_RS09420	2043041..2045011	-	1971	WP_015674145.1	penicillin-binding protein 2	-
ASU1_RS09425	2045094..2045561	-	468	WP_005598965.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
ASU1_RS09430	2045584..2045898	-	315	WP_015674146.1	ribosome silencing factor	-
ASU1_RS09435	2046038..2046346	-	309	WP_015674147.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
ASU1_RS09440	2046330..2046692	-	363	WP_015674148.1	hypothetical protein	Toxin
ASU1_RS09445	2046866..2049460	-	2595	WP_039195494.1	DNA mismatch repair protein MutS	-
ASU1_RS09450	2049626..2050375	-	750	WP_015674150.1	amino acid ABC transporter ATP-binding protein	-
ASU1_RS09455	2050397..2051113	-	717	WP_005605560.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-86)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14051.07 Da
• Isoelectric Point: 10.2511

>T48491 WP_015674148.1 NZ_CP009159:c2046692-2046330 [Actinobacillus suis ATCC 33415]
VKLVFVELPPFERYRQKHLSDEEYRHFQNELLENPNKGDLIQGTNGLRKIRIADMQRNKGKRGGARVIYYHIVNYSKILL
VTAYGKDEQSDLSNTERNMLANKVKRIVELEIRSSNGKTF

Nucleotide

• Length: 363 bp

>T48491 NZ_CP009159:c2046692-2046330 [Actinobacillus suis ATCC 33415]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTAGTAATGGGAAAACTTTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11652.60 Da
• Isoelectric Point: 8.5450

>AT48491 WP_015674147.1 NZ_CP009159:c2046346-2046038 [Actinobacillus suis ATCC 33415]
MGKLFDDLMEGLDAYEKHIQGKITLRTTVLEKPEPVELSPSEVKLIREKLNLSQAVFAHKLHTSVRTYQGWEQGKTKPNP
QAVLLLRMVDKSPQLLEQMAQL

Nucleotide

• Length: 309 bp

>AT48491 NZ_CP009159:c2046346-2046038 [Actinobacillus suis ATCC 33415]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGCCCGTCTGAAGTTAAATTGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACCAAACCGAATCCA
CAAGCGGTACTGTTGCTTCGTATGGTGGATAAATCGCCTCAGTTATTAGAACAAATGGCACAGCTCTAA

Structures

Toxin

• AlphaFold DB: K0G6E5