Detailed information of TA system TA048488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1273143..1273788
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0G4M5
• Locus tag: ASU1_RS05805
• Protein ID: WP_014991853.1
• Coordinates: 1273444..1273788 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASU1_RS05800
• Protein ID: WP_014991852.1
• Coordinates: 1273143..1273439 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS05775	1268320..1269324	-	1005	WP_005600359.1	Holliday junction branch migration DNA helicase RuvB	-
ASU1_RS05780	1269343..1269948	-	606	WP_014991848.1	Holliday junction branch migration protein RuvA	-
ASU1_RS05785	1270090..1270857	-	768	WP_014991849.1	spermidine/putrescine ABC transporter permease PotC	-
ASU1_RS05790	1270857..1271723	-	867	WP_014991850.1	spermidine/putrescine ABC transporter permease PotB	-
ASU1_RS05795	1271710..1272825	-	1116	WP_014991851.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
ASU1_RS05800	1273143..1273439	-	297	WP_014991852.1	helix-turn-helix domain-containing protein	Antitoxin
ASU1_RS05805	1273444..1273788	-	345	WP_014991853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASU1_RS05810	1273993..1276011	+	2019	WP_014991854.1	DNA helicase Rep	-
ASU1_RS05815	1276100..1276852	-	753	WP_014991855.1	TatD family hydrolase	-
ASU1_RS05820	1276946..1278097	-	1152	WP_039195223.1	methionine adenosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (39-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-83)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13348.34 Da
• Isoelectric Point: 7.9875

>T48488 WP_014991853.1 NZ_CP009159:c1273788-1273444 [Actinobacillus suis ATCC 33415]
MNSDEYLTFVEVGTFEEDRKQLMADDEYQLFQAYLLEHYELGDFISHTGGCQKIRWRLPENNKGKSAGVRIIYYVRSLTG
RIYLITMYGKSEKVNLNAREKAIMKKIISRLIGE

Nucleotide

• Length: 345 bp

>T48488 NZ_CP009159:c1273788-1273444 [Actinobacillus suis ATCC 33415]
ATGAATTCTGATGAATATTTAACCTTTGTTGAGGTTGGTACATTCGAGGAAGATCGTAAGCAATTAATGGCAGACGATGA
GTACCAATTATTCCAAGCATATTTATTAGAGCATTACGAATTAGGGGATTTTATTTCCCATACAGGTGGTTGCCAAAAGA
TCAGGTGGCGCTTACCGGAAAATAATAAGGGGAAAAGTGCTGGTGTTCGAATTATCTATTATGTTAGAAGTTTAACAGGG
CGAATTTACCTAATTACTATGTATGGAAAAAGCGAAAAAGTTAATCTAAATGCTCGAGAAAAAGCGATTATGAAGAAAAT
TATTTCGAGATTAATAGGAGAGTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11129.00 Da
• Isoelectric Point: 10.4778

>AT48488 WP_014991852.1 NZ_CP009159:c1273439-1273143 [Actinobacillus suis ATCC 33415]
MEKRLFERLVQGLEEMVAIEKGEVEVPSHRIHAHKIPNTKAIRTQHHLKQAEFAQLLGVSTNLVQSWEIGRRVPNGAARK
LLAIIEQQPNLVPLLQSL

Nucleotide

• Length: 297 bp

>AT48488 NZ_CP009159:c1273439-1273143 [Actinobacillus suis ATCC 33415]
ATGGAAAAACGTTTATTTGAGCGCCTTGTACAAGGATTAGAAGAAATGGTTGCTATTGAAAAAGGTGAGGTGGAAGTACC
ATCGCATCGCATCCATGCACATAAAATTCCAAATACTAAGGCGATTCGTACACAACACCATTTGAAACAGGCAGAATTTG
CACAGCTGTTAGGTGTCAGTACCAATTTGGTGCAATCTTGGGAAATCGGCAGACGAGTCCCTAATGGAGCGGCAAGAAAA
TTATTGGCGATTATCGAACAACAACCGAATTTAGTCCCTTTGCTACAATCTCTCTAA

Structures

Toxin

• AlphaFold DB: K0G4M5