Detailed information of TA system TA048483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1338532..1339182
• Replicon: chromosome
• Accession: NZ_CP009158
• Organism: Glaesserella parasuis strain SH03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S9ZYD2
• Locus tag: JL26_RS06665
• Protein ID: WP_021115585.1
• Coordinates: 1338532..1338870 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL26_RS06670
• Protein ID: WP_005710405.1
• Coordinates: 1338874..1339182 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL26_RS06625	1334118..1334492	+	375	WP_010786049.1	Soluble cytochrome b562	-
JL26_RS06630	1334632..1335566	+	935	Protein_1329	transposase	-
JL26_RS11795	1335521..1335787	+	267	Protein_1330	cation-transporting ATPase PacS	-
JL26_RS06640	1336254..1337693	-	1440	WP_038514496.1	glutamate--tRNA ligase	-
JL26_RS06665	1338532..1338870	+	339	WP_021115585.1	selenocysteine synthase	Toxin
JL26_RS06670	1338874..1339182	+	309	WP_005710405.1	helix-turn-helix domain-containing protein	Antitoxin
JL26_RS06675	1339383..1340543	+	1161	WP_010786053.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
JL26_RS06680	1340643..1341224	+	582	WP_021110392.1	type IV pilus biogenesis/stability protein PilW	-
JL26_RS06685	1341286..1342221	+	936	WP_005710401.1	KpsF/GutQ family sugar-phosphate isomerase	-
JL26_RS06690	1342221..1342709	+	489	WP_005710400.1	transcription elongation factor GreB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1334632..1335171	539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-86)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13258.29 Da
• Isoelectric Point: 10.0084

>T48483 WP_021115585.1 NZ_CP009158:1338532-1338870 [Glaesserella parasuis]
MNVAFVELPPFEEYRKKYLDDDSFRLLQNELLKFPDKGELIQGTGGLRKLRIVDIIRQKGKRGGARVIYYYYVRGKQVWL
FHAYNKNQQDDLSNEERVVLANTLSYLKSLVR

Nucleotide

• Length: 339 bp

>T48483 NZ_CP009158:1338532-1338870 [Glaesserella parasuis]
ATGAATGTCGCTTTTGTGGAGTTGCCGCCTTTTGAGGAATATAGAAAAAAGTATCTTGACGATGACTCATTTAGATTATT
ACAAAATGAGTTACTAAAATTTCCCGATAAGGGAGAGCTTATTCAAGGTACTGGGGGACTAAGAAAATTGCGTATTGTGG
ATATTATTAGACAAAAAGGAAAAAGAGGCGGAGCTAGGGTAATCTATTACTATTACGTGCGAGGGAAACAAGTTTGGTTG
TTCCACGCCTACAATAAAAACCAACAAGACGATTTATCTAATGAAGAACGTGTGGTTTTGGCGAATACATTAAGCTATTT
AAAATCTTTAGTGAGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11539.61 Da
• Isoelectric Point: 10.2919

>AT48483 WP_005710405.1 NZ_CP009158:1338874-1339182 [Glaesserella parasuis]
MGKLFNELMEGMQALELHLQGKITLKTTVVEVPDPIVITPEEVKAIRQKLNLSQAVFARKLHTSVRTYQGWEQGRTKPNP
QAVLLLKMVEKSPKTFEQIASI

Nucleotide

• Length: 309 bp

>AT48483 NZ_CP009158:1338874-1339182 [Glaesserella parasuis]
ATGGGAAAATTATTTAATGAACTAATGGAAGGGATGCAAGCATTAGAGTTACACTTACAAGGCAAAATTACACTAAAAAC
CACTGTTGTGGAAGTTCCTGATCCGATAGTGATTACCCCCGAAGAAGTGAAAGCTATTCGTCAGAAATTAAATCTTTCAC
AAGCCGTTTTTGCTCGTAAATTACACACTTCGGTGAGAACTTATCAAGGCTGGGAGCAAGGAAGAACAAAGCCCAATCCG
CAAGCGGTCTTACTATTAAAAATGGTTGAAAAATCGCCAAAAACATTTGAGCAAATTGCGAGCATTTAA

Structures

Toxin

• AlphaFold DB: A0A1S9ZYD2