Detailed information of TA system TA048481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 758742..759303
• Replicon: chromosome
• Accession: NZ_CP009158
• Organism: Glaesserella parasuis strain SH03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMA9
• Locus tag: JL26_RS03980
• Protein ID: WP_010786777.1
• Coordinates: 758742..759020 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: JL26_RS03985
• Protein ID: WP_005712616.1
• Coordinates: 759031..759303 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL26_RS03965	754532..755860	-	1329	WP_038515156.1	exodeoxyribonuclease VII large subunit	-
JL26_RS03970	755974..757236	-	1263	WP_010786776.1	serine hydroxymethyltransferase	-
JL26_RS03975	757583..758593	-	1011	WP_038513972.1	ornithine carbamoyltransferase	-
JL26_RS03980	758742..759020	+	279	WP_010786777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL26_RS03985	759031..759303	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
JL26_RS03990	759367..760587	+	1221	WP_038513975.1	ATP-dependent RNA helicase RhlB	-
JL26_RS03995	760597..760905	+	309	Protein_802	DUF853 family protein	-
JL26_RS04000	760977..762191	-	1215	WP_080716934.1	ATP-binding protein	-
JL26_RS04005	762396..762938	-	543	WP_021115958.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10278.66 Da
• Isoelectric Point: 10.0505

>T48481 WP_010786777.1 NZ_CP009158:758742-759020 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKVQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T48481 NZ_CP009158:758742-759020 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACTGCAGGTATTCAGGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATCTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAAGTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT48481 WP_005712616.1 NZ_CP009158:759031-759303 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT48481 NZ_CP009158:759031-759303 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGCGTCACTCGTGTCACGCTTTCTCGTATTTTAAATGGTAAAGCTGCAATTTCACCTGAAATGGCTCTTAGAT
TGAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: U4SMA9