Detailed information of TA system TA048480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 581511..582154
• Replicon: chromosome
• Accession: NZ_CP009158
• Organism: Glaesserella parasuis strain SH03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: JL26_RS03120
• Protein ID: WP_005714197.1
• Coordinates: 581511..581855 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL26_RS03125
• Protein ID: WP_016527809.1
• Coordinates: 581858..582154 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL26_RS03110	578115..579206	-	1092	WP_005714201.1	murein transglycosylase A	-
JL26_RS03115	579317..581296	-	1980	WP_038513834.1	exoribonuclease II	-
JL26_RS03120	581511..581855	+	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL26_RS03125	581858..582154	+	297	WP_016527809.1	helix-turn-helix domain-containing protein	Antitoxin
JL26_RS03130	582211..583173	+	963	WP_038513837.1	WYL domain-containing protein	-
JL26_RS03135	583249..584022	+	774	WP_035519505.1	PD-(D/E)XK nuclease family protein	-
JL26_RS03140	584039..584686	+	648	WP_016527812.1	hypothetical protein	-
JL26_RS03145	584965..585681	+	717	WP_035519506.1	hypothetical protein	-
JL26_RS03150	585726..586695	+	970	Protein_629	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T48480 WP_005714197.1 NZ_CP009158:581511-581855 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T48480 NZ_CP009158:581511-581855 [Glaesserella parasuis]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCTCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11196.11 Da
• Isoelectric Point: 8.8246

>AT48480 WP_016527809.1 NZ_CP009158:581858-582154 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPELIPVLQGL

Nucleotide

• Length: 297 bp

>AT48480 NZ_CP009158:581858-582154 [Glaesserella parasuis]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAACCTTGTGCAGTCGTGGGAAATTGGCAGACGAACCCCAAATGGCGCTGCACGGAAA
TTGCTTGCGATTATTGAACAACAGCCAGAACTTATCCCTGTATTACAAGGGCTTTAG

Structures

Toxin

• AlphaFold DB: U4SMB1