Detailed information of TA system TA048446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1136276..1136894
• Replicon: chromosome
• Accession: NZ_CP009144
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: DU99_RS05315
• Protein ID: WP_010969011.1
• Coordinates: 1136598..1136894 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DU99_RS05310
• Protein ID: WP_010969010.1
• Coordinates: 1136276..1136584 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS05265	1131356..1131676	-	321	WP_003527320.1	YnfA family protein	-
DU99_RS36055	1131751..1131924	-	174	WP_010969006.1	hypothetical protein	-
DU99_RS36060	1131938..1132111	-	174	WP_003527318.1	hypothetical protein	-
DU99_RS05280	1132346..1133047	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
DU99_RS05285	1133271..1133612	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
DU99_RS05290	1134197..1134463	+	267	WP_003527312.1	sel1 repeat family protein	-
DU99_RS05295	1134485..1135606	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
DU99_RS05305	1136035..1136265	-	231	WP_026029805.1	hypothetical protein	-
DU99_RS05310	1136276..1136584	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
DU99_RS05315	1136598..1136894	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DU99_RS05320	1136942..1137247	-	306	WP_003527305.1	hypothetical protein	-
DU99_RS05325	1137575..1138612	+	1038	WP_003527304.1	porin	-
DU99_RS05330	1138722..1139789	-	1068	WP_014526650.1	site-specific integrase	-
DU99_RS05335	1139830..1140879	-	1050	WP_010969013.1	porin	-
DU99_RS05340	1141093..1141581	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T48446 WP_010969011.1 NZ_CP009144:c1136894-1136598 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T48446 NZ_CP009144:c1136894-1136598 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT48446 WP_010969010.1 NZ_CP009144:c1136584-1136276 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT48446 NZ_CP009144:c1136584-1136276 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61