Detailed information of TA system TA048431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 499998..500500
• Replicon: chromosome
• Accession: NZ_CP009125
• Organism: Pectobacterium atrosepticum strain 21A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q6DA20
• Locus tag: GZ59_RS02235
• Protein ID: WP_011092065.1
• Coordinates: 499998..500252 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q6DA19
• Locus tag: GZ59_RS02240
• Protein ID: WP_011092066.1
• Coordinates: 500249..500500 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ59_RS02215	495172..496434	-	1263	WP_039289755.1	L-rhamnose isomerase	-
GZ59_RS02220	496431..497921	-	1491	WP_039289758.1	rhamnulokinase	-
GZ59_RS02225	498238..499077	+	840	WP_011092063.1	HTH-type transcriptional activator RhaS	-
GZ59_RS02230	499102..499956	+	855	WP_039289761.1	helix-turn-helix domain-containing protein	-
GZ59_RS02235	499998..500252	-	255	WP_011092065.1	Txe/YoeB family addiction module toxin	Toxin
GZ59_RS02240	500249..500500	-	252	WP_011092066.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
GZ59_RS02245	500575..502284	-	1710	WP_011092067.1	ABC transporter ATP-binding protein	-
GZ59_RS02250	502281..503147	-	867	WP_011092068.1	ABC transporter permease	-
GZ59_RS02255	503140..504099	-	960	WP_011092069.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10141.67 Da
• Isoelectric Point: 8.8560

>T48431 WP_011092065.1 NZ_CP009125:c500252-499998 [Pectobacterium atrosepticum]
VKLIWSEEAWEDYLYWQDIDKRTVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEAHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T48431 NZ_CP009125:c500252-499998 [Pectobacterium atrosepticum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGAACGGTCAAAAAGAT
CAATGAATTAATAAAAGAGACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGCACATCGTCTGGTGTATGCCATCACAGATGATGCCATGATGATCGCCGCATGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9397.66 Da
• Isoelectric Point: 4.6119

>AT48431 WP_011092066.1 NZ_CP009125:c500500-500249 [Pectobacterium atrosepticum]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNSEERD
IIE

Nucleotide

• Length: 252 bp

>AT48431 NZ_CP009125:c500500-500249 [Pectobacterium atrosepticum]
ATGCGTACAATTAGCTACAGCGAAGCCCGTCAAAATCTATCAGCGACAATGATGAAAACCGTCGAAGATCGTGCTCCCAT
TCTGATCACTCGACAGAATGGTGAGGCCTGCGTGCTTATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACAGCATATT
TACTGCGTTCACCAGCAAATGCAAAAAGGCTGATGAATTCAATTGAAAGCCTTAAGGCTGGCAATAGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q6DA20

Antitoxin

• AlphaFold DB: Q6DA19