Detailed information of TA system TA048425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 173815..174338
• Replicon: chromosome
• Accession: NZ_CP009120
• Organism: Staphylococcus pseudintermedius strain 5912

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: SPS5912_RS00755
• Protein ID: WP_063278654.1
• Coordinates: 174072..174338 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A3D8YKM0
• Locus tag: SPS5912_RS00750
• Protein ID: WP_014612814.1
• Coordinates: 173815..174072 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPS5912_RS00735	170167..170973	+	807	WP_114281618.1	arylamine N-acetyltransferase	-
SPS5912_RS00740	171267..171761	-	495	WP_015729749.1	membrane protein	-
SPS5912_RS00745	172021..173621	+	1601	Protein_146	hypothetical protein	-
SPS5912_RS00750	173815..174072	+	258	WP_014612814.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPS5912_RS00755	174072..174338	+	267	WP_063278654.1	Txe/YoeB family addiction module toxin	Toxin
SPS5912_RS00760	174689..174790	-	102	WP_019169745.1	DUF2648 domain-containing protein	-
SPS5912_RS00765	174804..176297	-	1494	WP_014612817.1	malate dehydrogenase (quinone)	-
SPS5912_RS12055	177054..177155	+	102	Protein_151	KxYKxGKxW signal peptide domain-containing protein	-
SPS5912_RS12060	178588..179025	-	438	WP_206605424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10539.11 Da
• Isoelectric Point: 10.2281

>T48425 WP_063278654.1 NZ_CP009120:174072-174338 [Staphylococcus pseudintermedius]
MGNYSVLIKNSAKNDLKKIKKPLLKEQFSKIIEVLKINPYEASQSFEKLQPKHSARYSRRLNHQHRVVYTVDDEKREVYI
FSAWSHYE

Nucleotide

• Length: 267 bp

>T48425 NZ_CP009120:174072-174338 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTTTTAATTAAGAATTCAGCCAAAAATGACTTAAAAAAAATTAAAAAGCCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCACATTATGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9866.87 Da
• Isoelectric Point: 4.1100

>AT48425 WP_014612814.1 NZ_CP009120:173815-174072 [Staphylococcus pseudintermedius]
MIIKTPTNARKEFYELLKQVNDNHEPIIISGSKSENNAVIVSQEDWDSIQETLFLEYTGTMDKVRAREKDDSGYTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT48425 NZ_CP009120:173815-174072 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATAGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3D8YKM0