Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3114565..3115211 Replicon chromosome
Accession NZ_CP009114
Organism Klebsiella pneumoniae strain carbapenem-resistant blaNDM-1

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag FH42_RS15800 Protein ID WP_004174016.1
Coordinates 3114864..3115211 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag FH42_RS15795 Protein ID WP_019725405.1
Coordinates 3114565..3114864 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH42_RS15775 3110771..3111529 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
FH42_RS15780 3111579..3112409 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
FH42_RS15785 3112460..3112789 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
FH42_RS15790 3112994..3114502 + 1509 WP_019704656.1 glycerol-3-phosphate dehydrogenase -
FH42_RS15795 3114565..3114864 - 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
FH42_RS15800 3114864..3115211 - 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FH42_RS15805 3115387..3117834 - 2448 WP_002920561.1 glycogen phosphorylase -
FH42_RS15810 3117852..3119285 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T48416 WP_004174016.1 NZ_CP009114:c3115211-3114864 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T48416 NZ_CP009114:c3115211-3114864 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT48416 WP_019725405.1 NZ_CP009114:c3114864-3114565 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT48416 NZ_CP009114:c3114864-3114565 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References