Detailed information of TA system TA048401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4314490..4315289
• Replicon: chromosome
• Accession: NZ_CP009106
• Organism: Escherichia coli strain 94-3024

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: HW43_RS21330
• Protein ID: WP_021293021.1
• Coordinates: 4314490..4314954 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: HW43_RS21335
• Protein ID: WP_001307405.1
• Coordinates: 4314954..4315289 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW43_RS21300	4309491..4309925	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
HW43_RS21305	4309943..4310821	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HW43_RS21310	4310811..4311590	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HW43_RS21315	4311601..4312074	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HW43_RS21320	4312097..4313377	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HW43_RS21325	4313626..4314435	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HW43_RS21330	4314490..4314954	-	465	WP_021293021.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HW43_RS21335	4314954..4315289	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HW43_RS21340	4315438..4317009	-	1572	WP_001273741.1	galactarate dehydratase	-
HW43_RS21350	4317384..4318718	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
HW43_RS21355	4318734..4319504	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T48401 WP_021293021.1 NZ_CP009106:c4314954-4314490 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Nucleotide

• Length: 465 bp

>T48401 NZ_CP009106:c4314954-4314490 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT48401 WP_001307405.1 NZ_CP009106:c4315289-4314954 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT48401 NZ_CP009106:c4315289-4314954 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7