Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2925133..2925635 Replicon chromosome
Accession NZ_CP009106
Organism Escherichia coli strain 94-3024

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HW43_RS14455 Protein ID WP_000767829.1
Coordinates 2925133..2925387 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HW43_RS14460 Protein ID WP_001259255.1
Coordinates 2925384..2925635 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW43_RS14430 2920148..2920375 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HW43_RS14435 2920389..2921447 - 1059 WP_000492340.1 transport protein YeeE -
HW43_RS14440 2921626..2922984 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HW43_RS27760 2922974..2923036 - 63 WP_010723108.1 membrane protein YoeI -
HW43_RS14445 2923251..2924180 - 930 WP_000803351.1 LysR family transcriptional regulator -
HW43_RS14450 2924226..2925050 - 825 WP_000754749.1 SDR family oxidoreductase -
HW43_RS14455 2925133..2925387 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HW43_RS14460 2925384..2925635 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HW43_RS26050 2925918..2925968 + 51 WP_001364200.1 his operon leader peptide -
HW43_RS14465 2926114..2927013 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HW43_RS14470 2927019..2928323 + 1305 WP_021293346.1 histidinol dehydrogenase -
HW43_RS14475 2928320..2929390 + 1071 WP_000108973.1 histidinol-phosphate transaminase -
HW43_RS14480 2929390..2930457 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T48396 WP_000767829.1 NZ_CP009106:c2925387-2925133 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T48396 NZ_CP009106:c2925387-2925133 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT48396 WP_001259255.1 NZ_CP009106:c2925635-2925384 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT48396 NZ_CP009106:c2925635-2925384 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References