Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3011408..3011910 Replicon chromosome
Accession NZ_CP009104
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HW42_RS15155 Protein ID WP_000767829.1
Coordinates 3011408..3011662 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HW42_RS15160 Protein ID WP_001259255.1
Coordinates 3011659..3011910 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS15130 3006423..3006650 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HW42_RS15135 3006664..3007722 - 1059 WP_000492340.1 transport protein YeeE -
HW42_RS15140 3007901..3009259 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HW42_RS27895 3009249..3009311 - 63 WP_010723108.1 membrane protein YoeI -
HW42_RS15145 3009526..3010455 - 930 WP_000803351.1 LysR family transcriptional regulator -
HW42_RS15150 3010501..3011325 - 825 WP_039264492.1 SDR family oxidoreductase -
HW42_RS15155 3011408..3011662 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HW42_RS15160 3011659..3011910 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HW42_RS26250 3012193..3012243 + 51 WP_001364200.1 his operon leader peptide -
HW42_RS15165 3012389..3013288 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HW42_RS15170 3013294..3014598 + 1305 WP_001542955.1 histidinol dehydrogenase -
HW42_RS15175 3014595..3015665 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
HW42_RS15180 3015665..3016732 + 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T48371 WP_000767829.1 NZ_CP009104:c3011662-3011408 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T48371 NZ_CP009104:c3011662-3011408 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT48371 WP_001259255.1 NZ_CP009104:c3011910-3011659 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT48371 NZ_CP009104:c3011910-3011659 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References