Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1050050..1050591 Replicon chromosome
Accession NZ_CP009104
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag HW42_RS05050 Protein ID WP_039264364.1
Coordinates 1050050..1050328 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HW42_RS05055 Protein ID WP_000729704.1
Coordinates 1050331..1050591 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS05035 1047632..1048210 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HW42_RS05040 1048416..1049183 + 768 WP_000333380.1 class II glutamine amidotransferase -
HW42_RS05045 1049154..1049894 - 741 WP_001225679.1 murein L,D-transpeptidase -
HW42_RS05050 1050050..1050328 - 279 WP_039264364.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HW42_RS05055 1050331..1050591 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HW42_RS05060 1050777..1051550 + 774 WP_000543899.1 C40 family peptidase -
HW42_RS05065 1051607..1051963 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HW42_RS05070 1051956..1052234 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HW42_RS05075 1052339..1054051 - 1713 Protein_930 flagellar biosynthesis protein FlhA -
HW42_RS05080 1054023..1054808 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10786.52 Da        Isoelectric Point: 9.9107

>T48359 WP_039264364.1 NZ_CP009104:c1050328-1050050 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHNDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T48359 NZ_CP009104:c1050328-1050050 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAATGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT48359 WP_000729704.1 NZ_CP009104:c1050591-1050331 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT48359 NZ_CP009104:c1050591-1050331 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References