Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976607..2977255 Replicon chromosome
Accession NZ_CP009101
Organism Mycobacterium tuberculosis strain ZMC13-88

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag JE53_RS14035 Protein ID WP_003899414.1
Coordinates 2976607..2976930 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag JE53_RS14040 Protein ID WP_003899415.1
Coordinates 2977010..2977255 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE53_RS14015 2972181..2973442 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JE53_RS14020 2973816..2975255 - 1440 WP_003901443.1 phage major capsid protein -
JE53_RS14025 2975263..2975796 - 534 WP_003899412.1 HK97 family phage prohead protease -
JE53_RS21605 2975949..2976440 - 492 WP_003900541.1 phage terminase small subunit P27 family -
JE53_RS14035 2976607..2976930 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
JE53_RS14040 2977010..2977255 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
JE53_RS14045 2977252..2978679 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
JE53_RS14050 2978681..2979073 - 393 WP_003899417.1 DUF2742 domain-containing protein -
JE53_RS14055 2979070..2979330 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
JE53_RS14060 2979347..2979709 - 363 WP_003900543.1 hypothetical protein -
JE53_RS14065 2979712..2980839 - 1128 WP_003899420.1 site-specific integrase -
JE53_RS14070 2980984..2981211 - 228 WP_003899421.1 hypothetical protein -
JE53_RS21610 2981208..2981597 - 390 WP_003899422.1 hypothetical protein -
JE53_RS14075 2981503..2981775 + 273 WP_003900544.1 hypothetical protein -
JE53_RS14080 2981874..2982107 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970573..2980839 10266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-104)

Antitoxin

Antitoxin CbeA

(18-116)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T48342 WP_003899414.1 NZ_CP009101:c2976930-2976607 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T48342 NZ_CP009101:c2976930-2976607 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT48342 WP_003899415.1 NZ_CP009101:c2977255-2977010 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT48342 NZ_CP009101:c2977255-2977010 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References