Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2266833..2267752 Replicon chromosome
Accession NZ_CP009101
Organism Mycobacterium tuberculosis strain ZMC13-88

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JE53_RS21355 Protein ID WP_003900449.1
Coordinates 2267147..2267752 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JE53_RS10615 Protein ID WP_003410124.1
Coordinates 2266833..2267138 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE53_RS10585 2261843..2263099 - 1257 WP_003410095.1 HNH endonuclease -
JE53_RS10590 2263453..2264028 + 576 WP_003410100.1 hypothetical protein -
JE53_RS10595 2264025..2265065 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JE53_RS10600 2265307..2266026 + 720 WP_003410108.1 DUF433 domain-containing protein -
JE53_RS10605 2266016..2266432 + 417 WP_003410114.1 hypothetical protein -
JE53_RS10610 2266448..2266748 - 301 Protein_2085 hypothetical protein -
JE53_RS10615 2266833..2267138 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JE53_RS21355 2267147..2267752 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JE53_RS10625 2267777..2268136 - 360 WP_003410131.1 hypothetical protein -
JE53_RS10630 2268296..2268754 - 459 Protein_2089 hypothetical protein -
JE53_RS10635 2268751..2270268 - 1518 Protein_2090 DEAD/DEAH box helicase -
JE53_RS10640 2270778..2271776 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T48332 WP_003900449.1 NZ_CP009101:c2267752-2267147 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T48332 NZ_CP009101:c2267752-2267147 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT48332 WP_003410124.1 NZ_CP009101:c2267138-2266833 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT48332 NZ_CP009101:c2267138-2266833 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References