48233

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 15512..16158 Replicon plasmid unnamed_1
Accession NZ_CP009073
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID A0A7R6ZH56
Locus tag DR76_RS25500 Protein ID WP_000269913.1
Coordinates 15512..15859 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag DR76_RS25505 Protein ID WP_001259436.1
Coordinates 15859..16158 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS25470 10712..11239 - 528 WP_000972103.1 tail fiber assembly protein -
DR76_RS26045 11241..12167 - 927 Protein_12 phage tail protein -
DR76_RS25480 12333..12887 + 555 WP_001199265.1 recombinase family protein -
DR76_RS25485 13213..14049 + 837 WP_001139598.1 hypothetical protein -
DR76_RS25490 14693..14980 + 288 WP_000356589.1 hypothetical protein -
DR76_RS25495 15004..15267 + 264 WP_000424604.1 hypothetical protein -
DR76_RS25500 15512..15859 + 348 WP_000269913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DR76_RS25505 15859..16158 + 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
DR76_RS25510 16322..16702 + 381 WP_000061764.1 hypothetical protein -
DR76_RS25515 16766..17038 + 273 WP_000148349.1 helix-turn-helix domain-containing protein -
DR76_RS25520 17649..17837 + 189 WP_000986262.1 hypothetical protein -
DR76_RS25525 17848..18990 - 1143 WP_000854799.1 ORF6N domain-containing protein -
DR76_RS25530 18987..19178 - 192 WP_000183352.1 hypothetical protein -
DR76_RS25535 19752..20324 + 573 WP_000137931.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48488 48488


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13486.53 Da        Isoelectric Point: 9.8723

>T48233 WP_000269913.1 NZ_CP009073:15512-15859 [Escherichia coli ATCC 25922]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

>T48233 NZ_CP009073:15512-15859 [Escherichia coli ATCC 25922]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT48233 WP_001259436.1 NZ_CP009073:15859-16158 [Escherichia coli ATCC 25922]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp

>AT48233 NZ_CP009073:15859-16158 [Escherichia coli ATCC 25922]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7R6ZH56

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References