Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3591212..3591774 Replicon chromosome
Accession NZ_CP009072
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag DR76_RS17590 Protein ID WP_000605675.1
Coordinates 3591212..3591490 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag DR76_RS17595 Protein ID WP_000781370.1
Coordinates 3591490..3591774 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS17565 3586798..3587229 - 432 WP_000152307.1 peroxiredoxin OsmC -
DR76_RS17570 3587574..3587789 + 216 WP_000495766.1 biofilm-dependent modulation protein -
DR76_RS17575 3587891..3588028 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
DR76_RS17580 3588185..3589882 + 1698 WP_000433460.1 malate dehydrogenase -
DR76_RS17585 3590016..3591026 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
DR76_RS17590 3591212..3591490 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DR76_RS17595 3591490..3591774 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
DR76_RS17600 3592003..3592656 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
DR76_RS17605 3592649..3593533 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
DR76_RS17610 3593546..3596593 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T48227 WP_000605675.1 NZ_CP009072:3591212-3591490 [Escherichia coli ATCC 25922]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T48227 NZ_CP009072:3591212-3591490 [Escherichia coli ATCC 25922]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT48227 WP_000781370.1 NZ_CP009072:3591490-3591774 [Escherichia coli ATCC 25922]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT48227 NZ_CP009072:3591490-3591774 [Escherichia coli ATCC 25922]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References