Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2061338..2062065 Replicon chromosome
Accession NZ_CP009072
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag DR76_RS09965 Protein ID WP_000547555.1
Coordinates 2061338..2061649 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag DR76_RS09970 Protein ID WP_000126293.1
Coordinates 2061646..2062065 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS09935 2056479..2058188 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
DR76_RS09940 2058198..2058740 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
DR76_RS09945 2058740..2059507 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
DR76_RS09950 2059504..2059914 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
DR76_RS09955 2059907..2060377 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
DR76_RS09960 2060420..2061175 + 756 WP_001305268.1 hypothetical protein -
DR76_RS09965 2061338..2061649 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
DR76_RS09970 2061646..2062065 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
DR76_RS09975 2062157..2062585 - 429 WP_000536064.1 hypothetical protein -
DR76_RS09980 2062970..2063497 + 528 WP_001078777.1 electron transport protein HydN -
DR76_RS09985 2063650..2065902 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T48209 WP_000547555.1 NZ_CP009072:2061338-2061649 [Escherichia coli ATCC 25922]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T48209 NZ_CP009072:2061338-2061649 [Escherichia coli ATCC 25922]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT48209 WP_000126293.1 NZ_CP009072:2061646-2062065 [Escherichia coli ATCC 25922]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT48209 NZ_CP009072:2061646-2062065 [Escherichia coli ATCC 25922]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References