Detailed information of TA system TA048206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1469684..1470483
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: DR76_RS07065
• Protein ID: WP_000347252.1
• Coordinates: 1469684..1470148 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: DR76_RS07070
• Protein ID: WP_001296435.1
• Coordinates: 1470148..1470483 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS07035	1464685..1465119	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
DR76_RS07040	1465137..1466015	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
DR76_RS07045	1466005..1466784	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
DR76_RS07050	1466795..1467268	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
DR76_RS07055	1467291..1468571	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
DR76_RS07060	1468820..1469629	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
DR76_RS07065	1469684..1470148	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
DR76_RS07070	1470148..1470483	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
DR76_RS07075	1470632..1472203	-	1572	WP_001273940.1	galactarate dehydratase	-
DR76_RS07080	1472578..1473912	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
DR76_RS07085	1473928..1474698	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T48206 WP_000347252.1 NZ_CP009072:c1470148-1469684 [Escherichia coli ATCC 25922]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T48206 NZ_CP009072:c1470148-1469684 [Escherichia coli ATCC 25922]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT48206 WP_001296435.1 NZ_CP009072:c1470483-1470148 [Escherichia coli ATCC 25922]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT48206 NZ_CP009072:c1470483-1470148 [Escherichia coli ATCC 25922]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5