Detailed information of TA system TA048204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 631772..632374
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DR76_RS02915
• Protein ID: WP_000897302.1
• Coordinates: 632063..632374 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DR76_RS02910
• Protein ID: WP_000356397.1
• Coordinates: 631772..632062 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS02880	627845..628747	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DR76_RS02885	628744..629379	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DR76_RS02890	629376..630305	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
DR76_RS02895	630521..630739	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
DR76_RS02900	631135..631413	-	279	WP_001296612.1	hypothetical protein	-
DR76_RS02910	631772..632062	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DR76_RS02915	632063..632374	-	312	WP_000897302.1	hypothetical protein	Toxin
DR76_RS02920	632604..633512	+	909	WP_001305059.1	alpha/beta hydrolase	-
DR76_RS02925	633576..634517	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DR76_RS02930	634562..634999	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DR76_RS02935	634996..635868	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DR76_RS02940	635862..636461	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T48204 WP_000897302.1 NZ_CP009072:c632374-632063 [Escherichia coli ATCC 25922]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T48204 NZ_CP009072:c632374-632063 [Escherichia coli ATCC 25922]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT48204 WP_000356397.1 NZ_CP009072:c632062-631772 [Escherichia coli ATCC 25922]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT48204 NZ_CP009072:c632062-631772 [Escherichia coli ATCC 25922]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416