Detailed information of TA system TA048199

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21628..22054
• Replicon: plasmid p15648-3
• Accession: NZ_CP009053
• Organism: Escherichia coli NCCP15648

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A610_RS29555
• Protein ID: WP_001388592.1
• Coordinates: 21628..21786 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	21830..22054 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A610_RS29515	17016..17243	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
A610_RS29520	17331..18023	-	693	WP_000332477.1	PAS domain-containing protein	-
A610_RS29525	18217..18600	-	384	WP_001151566.1	relaxosome protein TraM	-
A610_RS29530	18940..19530	+	591	WP_176453891.1	transglycosylase SLT domain-containing protein	-
A610_RS29540	19825..20646	-	822	WP_001234482.1	DUF945 domain-containing protein	-
A610_RS30360	20825..21390	+	566	Protein_33	hypothetical protein	-
A610_RS29555	21628..21786	-	159	WP_001388592.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	21830..22054	-	225	NuclAT_0	-	Antitoxin
-	21830..22054	-	225	NuclAT_0	-	Antitoxin
-	21830..22054	-	225	NuclAT_0	-	Antitoxin
-	21830..22054	-	225	NuclAT_0	-	Antitoxin
A610_RS29560	21866..22045	+	180	WP_001309233.1	hypothetical protein	-
A610_RS29565	22066..22668	-	603	Protein_36	plasmid SOS inhibition protein A	-
A610_RS29585	23768..25435	+	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
A610_RS30555	25572..25688	+	117	Protein_38	pilus protein	-
A610_RS29595	25774..25956	-	183	Protein_39	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	pic / aap/aspU / agg3A / agg3B / agg3C / agg3D	1..78111	78111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6135.36 Da
• Isoelectric Point: 10.1101

>T48199 WP_001388592.1 NZ_CP009053:c21786-21628 [Escherichia coli NCCP15648]
MKLPRSSLVWRVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T48199 NZ_CP009053:c21786-21628 [Escherichia coli NCCP15648]
ATGAAACTACCACGAAGTTCCCTTGTCTGGCGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT48199 NZ_CP009053:c22054-21830 [Escherichia coli NCCP15648]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTGTATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin